Quantitative characterization of 3D bioprinted structural elements under cell generated forces.

Autor: Morley CD; University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA., Ellison ST; University of Florida, Herbert Wertheim College of Engineering, Department of Materials Science and Engineering, Gainesville, FL, 32611, USA., Bhattacharjee T; Princeton University, Department of Chemical and Biological Engineering, Princeton, NJ, 08540, USA., O'Bryan CS; University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA., Zhang Y; University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA., Smith KF; University of Florida, Herbert Wertheim College of Engineering, Department of Materials Science and Engineering, Gainesville, FL, 32611, USA., Kabb CP; University of Florida, George and Josephine Butler Polymer Research Laboratory, Center for Macromolecular Science and Engineering, Department of Chemistry, Gainesville, FL, 32611, USA., Sebastian M; Division of Neuro-Oncology, Preston A. Wells, Jr. Center for Brain Tumor Therapy, Lillian S. Wells Department of Neurosurgery, University of Florida, Gainesville, FL, 32611, USA., Moore GL; University of Florida, Brain Tumor Immunotherapy Program, Preston A. Wells Jr. Center for Brain Tumor Therapy, Lillian S. Wells Department of Neurosurgery, Gainesville, FL, 32611, USA., Schulze KD; Auburn University, Department of Mechanical Engineering, Auburn, AL, 36849, USA., Niemi S; University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA., Sawyer WG; University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA.; University of Florida, Herbert Wertheim College of Engineering, Department of Materials Science and Engineering, Gainesville, FL, 32611, USA., Tran DD; Division of Neuro-Oncology, Preston A. Wells, Jr. Center for Brain Tumor Therapy, Lillian S. Wells Department of Neurosurgery, University of Florida, Gainesville, FL, 32611, USA., Mitchell DA; University of Florida, Brain Tumor Immunotherapy Program, Preston A. Wells Jr. Center for Brain Tumor Therapy, Lillian S. Wells Department of Neurosurgery, Gainesville, FL, 32611, USA., Sumerlin BS; University of Florida, George and Josephine Butler Polymer Research Laboratory, Center for Macromolecular Science and Engineering, Department of Chemistry, Gainesville, FL, 32611, USA., Flores CT; University of Florida, Brain Tumor Immunotherapy Program, Preston A. Wells Jr. Center for Brain Tumor Therapy, Lillian S. Wells Department of Neurosurgery, Gainesville, FL, 32611, USA., Angelini TE; University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA. t.e.angelini@ufl.edu.; University of Florida, Herbert Wertheim College of Engineering, Department of Materials Science and Engineering, Gainesville, FL, 32611, USA. t.e.angelini@ufl.edu.; University of Florida, Herbert Wertheim College of Engineering, J. Crayton Pruitt Family Department of Biomedical Engineering, Gainesville, FL, 32611, USA. t.e.angelini@ufl.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2019 Jul 10; Vol. 10 (1), pp. 3029. Date of Electronic Publication: 2019 Jul 10.
DOI: 10.1038/s41467-019-10919-1
Abstrakt: With improving biofabrication technology, 3D bioprinted constructs increasingly resemble real tissues. However, the fundamental principles describing how cell-generated forces within these constructs drive deformations, mechanical instabilities, and structural failures have not been established, even for basic biofabricated building blocks. Here we investigate mechanical behaviours of 3D printed microbeams made from living cells and extracellular matrix, bioprinting these simple structural elements into a 3D culture medium made from packed microgels, creating a mechanically controlled environment that allows the beams to evolve under cell-generated forces. By varying the properties of the beams and the surrounding microgel medium, we explore the mechanical behaviours exhibited by these structures. We observe buckling, axial contraction, failure, and total static stability, and we develop mechanical models of cell-ECM microbeam mechanics. We envision these models and their generalizations to other fundamental 3D shapes to facilitate the predictable design of biofabricated structures using simple building blocks in the future.
Databáze: MEDLINE