Molecular Mechanisms Driving Switch Behavior in Xylem Cell Differentiation.

Autor:	Turco GM; Department of Plant Biology and Genome Center, University of California, Davis, Davis, CA 95616, USA., Rodriguez-Medina J; Department of Plant Biology and Genome Center, University of California, Davis, Davis, CA 95616, USA., Siebert S; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA., Han D; Department of Plant Biology and Genome Center, University of California, Davis, Davis, CA 95616, USA., Valderrama-Gómez MÁ; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA 95616, USA., Vahldick H; Department of Plant Biology and Genome Center, University of California, Davis, Davis, CA 95616, USA., Shulse CN; Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA., Cole BJ; Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA., Juliano CE; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA., Dickel DE; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA., Savageau MA; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA 95616, USA; Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA., Brady SM; Department of Plant Biology and Genome Center, University of California, Davis, Davis, CA 95616, USA. Electronic address: sbrady@ucdavis.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2019 Jul 09; Vol. 28 (2), pp. 342-351.e4.
DOI:	10.1016/j.celrep.2019.06.041
Abstrakt:	Plant xylem cells conduct water and mineral nutrients. Although most plant cells are totipotent, xylem cells are unusual and undergo terminal differentiation. Many genes regulating this process are well characterized, including the Vascular-related NAC Domain 7 (VND7), MYB46, and MYB83 transcription factors, which are proposed to act in interconnected feedforward loops (FFLs). Less is known regarding the molecular mechanisms underlying the terminal transition to xylem cell differentiation. Here, we generate whole-root and single-cell data, which demonstrate that VND7 initiates sharp switching of root cells to xylem cell identity. Based on these data, we identified 4 candidate VND7 downstream target genes capable of generating this switch. Although MYB46 responds to VND7 induction, it is not among these targets. This system provides an important model to study the emergent properties that may give rise to totipotency relative to terminal differentiation and reveals xylem cell subtypes. (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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