The optoelectronic microrobot: A versatile toolbox for micromanipulation.

Autor:	Zhang S; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Scott EY; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Singh J; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Chen Y; State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, 510275 Guangzhou, China., Zhang Y; State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, 510275 Guangzhou, China., Elsayed M; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Chamberlain MD; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Shakiba N; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Adams K; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Institute of Medical Sciences, University of Toronto, Toronto, ON M5S 1A8, Canada., Yu S; State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, 510275 Guangzhou, China.; Photonics Group, Merchant Venturers School of Engineering, University of Bristol, BS8 1UB Bristol, United Kingdom., Morshead CM; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada.; Institute of Medical Sciences, University of Toronto, Toronto, ON M5S 1A8, Canada.; Department of Surgery, University of Toronto, Toronto, ON M5T 1P5, Canada., Zandstra PW; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada.; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.; The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.; Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada., Wheeler AR; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; aaron.wheeler@utoronto.ca.; Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jul 23; Vol. 116 (30), pp. 14823-14828. Date of Electronic Publication: 2019 Jul 09.
DOI:	10.1073/pnas.1903406116
Abstrakt:	Microrobotics extends the reach of human-controlled machines to submillimeter dimensions. We introduce a microrobot that relies on optoelectronic tweezers (OET) that is straightforward to manufacture, can take nearly any desirable shape or form, and can be programmed to carry out sophisticated, multiaxis operations. One particularly useful program is a serial combination of "load," "transport," and "deliver," which can be applied to manipulate a wide range of micrometer-dimension payloads. Importantly, microrobots programmed in this manner are much gentler on fragile mammalian cells than conventional OET techniques. The microrobotic system described here was demonstrated to be useful for single-cell isolation, clonal expansion, RNA sequencing, manipulation within enclosed systems, controlling cell-cell interactions, and isolating precious microtissues from heterogeneous mixtures. We propose that the optoelectronic microrobotic system, which can be implemented using a microscope and consumer-grade optical projector, will be useful for a wide range of applications in the life sciences and beyond. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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