In vivo kinetic study of materno-fetal fatty acid transfer in obese and normal weight pregnant women.

Autor: Gázquez A; Department of Physiology, School of Biology, Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital 'Virgen de la Arrixaca', University of Murcia, Murcia, Spain.; LMU - Ludwig-Maximilians-Universität Munich, Division of Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, Munich, Germany., Prieto-Sánchez MT; Obstetrics and Gynecology Service, Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital 'Virgen de la Arrixaca', University of Murcia, Murcia, Spain., Blanco-Carnero JE; Obstetrics and Gynecology Service, Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital 'Virgen de la Arrixaca', University of Murcia, Murcia, Spain., van Harskamp D; Department of Paediatrics, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands., Perazzolo S; Bioengineering Science Research Group, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK.; Institute for Life Sciences, University of Southampton, Southampton, UK., Oosterink JE; Department of Paediatrics, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands., Demmelmair H; LMU - Ludwig-Maximilians-Universität Munich, Division of Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, Munich, Germany., Schierbeek H; Department of Paediatrics, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands., Sengers BG; Bioengineering Science Research Group, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK.; Institute for Life Sciences, University of Southampton, Southampton, UK., Lewis RM; Institute for Life Sciences, University of Southampton, Southampton, UK.; University of Southampton, Faculty of Medicine, Southampton, UK., van Goudoever JB; Department of Paediatrics, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands.; Bioengineering Science Research Group, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK.; Institute for Life Sciences, University of Southampton, Southampton, UK.; University of Southampton, Faculty of Medicine, Southampton, UK.; Department of Paediatrics, Free University of Amsterdam, Amsterdam, The Netherlands., Koletzko B; LMU - Ludwig-Maximilians-Universität Munich, Division of Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, Munich, Germany., Larqué E; Department of Physiology, School of Biology, Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital 'Virgen de la Arrixaca', University of Murcia, Murcia, Spain.
Jazyk: angličtina
Zdroj: The Journal of physiology [J Physiol] 2019 Oct; Vol. 597 (19), pp. 4959-4973. Date of Electronic Publication: 2019 Jul 24.
DOI: 10.1113/JP278146
Abstrakt: Key Points: Placental structure and function can be modified as a result of maternal obesity affecting materno-fetal fatty acids (FA) transport. We report for the first time, in humans and in vivo, the kinetics of placental FA transfer in normo-weight and in normolipemic obese pregnant women using stable isotopes. The administration of different tracer FA with similar behaviour to the mother at different time points allows the collection of kinetic information on materno-fetal transfer of FA despite only one sample of placenta and cord can be collected per subject. Computational modelling showed a good fit to the data when considering all maternal plasma lipid classes but not when based only on non-esterified FA. The novel approach using multiple tracer FA administration combined with computational modelling shows a consistent time course of placental tracer FA and predicted total FA accumulation.
Abstract: We analyse for the first time the in vivo materno-fetal kinetic transfer of fatty acids (FA) labelled with stable isotopes in control and obese (OB) pregnant women. Labelled FA with a similar metabolism (stearic acid: 13 C-SA; palmitic acid: 13 C-PA; oleic acid: 13 C-OA) were orally administered at -4 h, -8 h and -12 h, respectively prior to elective caesarean section to 10 pregnant women with a body mass index >30 (OB) and 10 with a body mass index in the range 20-25 (NW). Placenta, venous and arterial cord blood were collected obtaining a wide range of FA enrichments. A combined experimental and computational modelling analysis was applied. FA fractional synthesis rate (FSR) in placenta was 11-12% h -1 . No differences were observed between NW and normo-lipidemic OB. It was not possible to estimate FA FSR in cord blood with this oral bolus dose approach. Computational modelling demonstrated a good fit to the data when all maternal plasma lipid classes were included but not with modelling based only on the non-esterified FA fraction. The estimated materno-fetal 13 C-FA transfer was ∼1%. In conclusion, our approach using multiple 13 C-FA tracers allowed us to estimated FSR in placental/maternal plasma but not in fetal/maternal compartments. Computational modelling showed a consistent time course of placental 13 C-FA transfer and predicted total fetal FA accumulation during the experiment. We conclude that, in addition to non-esterified FA fraction in the maternal circulation, maternal plasma very low-density lipoprotein and other lipoproteins are important contributors to placental FA transfer to the fetus.
(© 2019 The Authors. The Journal of Physiology © 2019 The Physiological Society.)
Databáze: MEDLINE
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