Selected CD molecules and the phagocytosis of microvesicles released from erythrocytes ex vivo.

Autor: Stachurska A; Department of Immunohematology, Centre of Postgraduate Medical Education, Warsaw, Poland., Dorman M; Department of Clinical Transfusiology, Military Institute of Medicine, Warsaw, Poland., Korsak J; Department of Clinical Transfusiology, Military Institute of Medicine, Warsaw, Poland., Gaweł D; Department of Biochemistry, Centre of Postgraduate Medical Education, Warsaw, Poland., Grzanka M; Department of Biochemistry, Centre of Postgraduate Medical Education, Warsaw, Poland., Trybus W; Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland., Fabijanska-Mitek J; Department of Immunohematology, Centre of Postgraduate Medical Education, Warsaw, Poland.
Jazyk: angličtina
Zdroj: Vox sanguinis [Vox Sang] 2019 Aug; Vol. 114 (6), pp. 576-587. Date of Electronic Publication: 2019 Jul 08.
DOI: 10.1111/vox.12819
Abstrakt: Background and Objectives: The accumulation of microvesicles in erythrocyte concentrates during storage or irradiation may be responsible for clinical symptoms such as inflammation, coagulation and immunization. Our aim was to determine whether any of the cluster of differentiation (CD) molecules responsible for important functions are present on microvesicles, and if their expression level is dependent on the storage period of erythrocyte concentrates.
Material and Methods: Erythrocyte microvesicles were isolated from 'fresh' (2 nd day) and 'old' (42 nd day) stored erythrocyte concentrates. Qualitative cytometric analysis of 0·5 µm, erythrocyte-derived, PS-exposing vesicles was performed using the annexin V-FITC, anti-CD235a-PE antibody and calibrated beads. The microvesicles were also visualized under a confocal microscope. The expression of the molecules CD235a, CD44, CD47, CD55, CD59 and of phosphatidylserine (PS) was compared using flow cytometry. Measurements of microvesicle phagocytosis by human monocytes were carried out using a flow cytometer and a confocal microscope.
Results: The analysis of the microvesicles with calibration beads allowed us to identify these structures with a diameter of about 0·5 µm in the 'fresh' and 'old' samples. At day 2, the microvesicles had elevated expression levels of CD47, reduced expression levels of PS, CD55 and CD59. The phagocytosis index was higher for the microvesicles isolated from the 42-day-old erythrocyte concentrates.
Conclusion: This research may bring us closer to understanding the factors responsible for erythrocyte ageing and to evaluate the quality of stored red blood concentrates intended for transfusion.
(© 2019 International Society of Blood Transfusion.)
Databáze: MEDLINE
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