Increased Fecal Lactobacillus Is Associated With a Positive Glucose Hydrogen Breath Test in Bangladeshi Children.

Autor: Donowitz JR; Division of Pediatric Infectious Diseases, Children's Hospital of Richmond at Virginia Commonwealth University, Richmond, Virginia.; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia., Parikh HI; School of Medicine Research Computing, University of Virginia, Charlottesville, Virginia., Taniuchi M; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia., Gilchrist CA; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia., Haque R; Division of Parasitology, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Kirkpatrick BD; Department of Microbiology and Molecular Genetics, and Vaccine Testing Center, University of Vermont College of Medicine, Burlington, Vermont., Alam M; Division of Parasitology, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Kakon SH; Division of Parasitology, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Islam BZ; James P. Grant School of Public Health, Brac University, Dhaka, Bangladesh., Afreen S; Division of Parasitology, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Kabir M; Division of Parasitology, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh., Nayak U; Center for Public Health Genomics, University of Virginia, Charlottesville, Charlottesville, Virginia., Colgate ER; Department of Microbiology and Molecular Genetics, and Vaccine Testing Center, University of Vermont College of Medicine, Burlington, Vermont., Carmolli MP; Department of Microbiology and Molecular Genetics, and Vaccine Testing Center, University of Vermont College of Medicine, Burlington, Vermont., Petri WA Jr; Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia.
Jazyk: angličtina
Zdroj: Open forum infectious diseases [Open Forum Infect Dis] 2019 Jun 01; Vol. 6 (7), pp. ofz266. Date of Electronic Publication: 2019 Jun 01 (Print Publication: 2019).
DOI: 10.1093/ofid/ofz266
Abstrakt: Background: Glucose hydrogen breath testing is a noninvasive test for small intestine bacterial overgrowth (SIBO). A positive glucose hydrogen breath test is common in children from low-income countries and has been found to be associated with malnutrition as measured by stunted growth. The microbiome associated with positive breath testing is relatively unstudied.
Methods: We performed 16 S V4 rDNA microbiome analysis on the stool of 90 Bangladeshi children aged 2 years from an impoverished neighborhood who were tested at the same time for SIBO by glucose hydrogen breath testing. Data were analyzed by linear discriminant analysis effect size with SIBO as the outcome. Any selected genera were tested individually by Wilcoxon's rank-sum test to ensure that linear discriminant analysis effect size results were not outlier-skewed.
Results: Linear discriminant analysis effect size analysis identified Lactobacillus (linear discriminate analysis score, 4.59; P = .03) as over-represented in 15 out of the 90 children who were SIBO positive.
Conclusions: These results suggest that glucose hydrogen breath test positivity in children from low-income settings may be due to an upper intestinal Lactobacillus bloom, potentially explaining the association of SIBO with the gut damage and inflammation that leads to malnutrition.
Databáze: MEDLINE
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