| Autor: |
Jofré I; Laboratory of Neurosciences and Biological Peptides (BIOREN-CEGIN-CEBIOR-UFRO) Medicine Faculty, Universidad de La Frontera, Francisco Salazar, 01145 Temuco, Chile., Cuevas M; Department of Physiology, University of Concepción, PO Box 160-C, Concepción, Chile., de Castro LS; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil., de Agostini Losano JD; Laboratório de Andrologia, Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Torres MA; Swine Research Center, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil., Alvear M; Department of Chemical Sciences and Natural Resources, Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, 01145 Temuco, Chile., Scheuermann E; Department of Chemical Engineering, Universidad de La Frontera, Francisco Salazar, 01145 Temuco, Chile., Andrade AFC; Swine Research Center, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil., Nichi M; Laboratório de Andrologia, Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Assumpção MEO; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil., Romero F; Laboratory of Neurosciences and Biological Peptides (BIOREN-CEGIN-CEBIOR-UFRO) Medicine Faculty, Universidad de La Frontera, Francisco Salazar, 01145 Temuco, Chile. |
| Abstrakt: |
The production of reactive oxygen species (ROS) in boar spermatozoa increases in refrigeration; this can have an impact on sperm quality and fertilization capacity. We evaluated the effect of polyphenol-rich aqueous extract of murtilla ( Ugni molinae Turcz) on boar sperm stored at 17°C in order to reduce oxidative stress and improve sperm quality in the long term. Five experiments were performed: first, characterization of the polyphenol content from five genotypes of murtilla; second, determination of the genotype with the best antioxidant effect (MT-Ex); third, the antioxidant capacity on O 2 - and lipid peroxidation; fourth, the influence of MT-Ex on motility, calcium movement, cAMP, and metabolic parameters; and fifth, analysis of long-term refrigeration. The average phenolic content was 344 ppm; gallic acid, catechin, quercetin, myricetin, and kaempferol were detected. All extracts evaluated presented a concentration-dependent antioxidant effect. MT-Ex reduces intracellular O 2 - /peroxides but low lipid peroxidation. MT-Ex in nonstimulated ROS conditions reduces sperm motility, mitochondrial membrane potential, cAMP, and ATP, but the succinate dehydrogenase activity remained normal; also, we observed a reduction in calcium movement in in vitro sperm capacitation. The long-term analyses showed that MT-Ex improved sperm motility decay and reduced membrane damage and ROS at 168 h. Based on this study, we propose MT-Ex as a supplement in semen extenders. |
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