Mapping of Novel Leaf Rust and Stem Rust Resistance Genes in the Portuguese Durum Wheat Landrace PI 192051.

Autor: Aoun M; Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108., Kolmer JA; United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, and Department of Plant Pathology, University of Minnesota, St. Paul 55108., Rouse MN; United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, and Department of Plant Pathology, University of Minnesota, St. Paul 55108., Elias EM; Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108., Breiland M; Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108., Bulbula WD; Ethiopian Institute of Agricultural Research, P.O. Box 32, Debre Zeit, Ethiopia., Chao S; SDA-ARS Cereal Crops Research Unit, Fargo, ND, 58102, and., Acevedo M; International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, 14853 ma934@cornell.edu.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2019 Aug 08; Vol. 9 (8), pp. 2535-2547. Date of Electronic Publication: 2019 Aug 08.
DOI: 10.1534/g3.119.400292
Abstrakt: Leaf rust caused by Puccinia triticina Erikss. ( Pt ) and stem rust caused by Puccinia graminis f. sp. tritic i Erikss. & E. Henn ( Pgt ) are serious constraints to production of durum wheat ( Triticum turgidum L). The objective of this study was to identify leaf rust resistance ( Lr ) and stem rust resistance ( Sr ) genes/QTL in Portuguese durum landrace PI 192051. Four Pt -isolates, representing three virulence phenotypes (BBBQJ, BBBSJ & EEEEE) and six Pgt -races TTKSK, JRCQC, TKTTF, QFCFC, TPMKC and TMLKC were used to evaluate 180 recombinant inbred lines (RILs) derived from the cross Rusty (rust susceptible) × PI 192051-1 (rust resistant) at the seedling stage. The RILs were also phenotyped at the adult-plant stage in a stem rust nursery in Ethiopia in 2017. The RILs were genotyped using the Illumina iSelect 9K wheat SNP array. PI 192051-1 carries a previously unidentified major Sr gene designated as QSr.ace-7A on chromosome arm 7AS and Lr gene Lr.ace-4A in the pericentromeric region of chromosome 4A. In addition, three minor Sr QTL QSr.ace-1A , QSr.ace-2B and QSr.ace-4A were mapped in PI 192051-1 on chromosomes 1AL, 2BL, and 4A, respectively Lr.ace-4A could be co-located or tightly linked to QSr.ace-4A Markers linked to the identified QTL/genes can be used for marker assisted selection. These findings enrich the genetic basis of rust resistance in both durum and common wheat.
(Copyright © 2019 Aoun et al.)
Databáze: MEDLINE
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