Human pegivirus-1 (HPgV-1) RNA prevalence and genotypes in volunteer blood donors from the Brazilian Amazon.

Autor: Slavov SN; Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil; Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil. Electronic address: svetoslav.slavov@hemocentro.fmrp.usp.br., Maraninchi Silveira R; Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil., Hespanhol MR; Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil., Sauvage V; Institut national de la transfusion Sanguine (INTS), département d'études des Agents transmissibles par le sang (DATS), Centre national de référence risques infectieux transfusionnels, 75015 Paris, France., Rodrigues ES; Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil., Fontanari Krause L; Laboratory of Biosciences, Franciscan University, 97010-030 Santa Maria, Rio Grande do Sul, Brazil., Bittencourt HT; Institute of Hematology and Hemotherapy of Amapá, 68900-074 Macapá, Amapá, Brazil., Caro V; Pole for Genotyping of Pathogens (PGP), Laboratory for Urgent Response to Biological Threats, Environment and Infectious Risks Research and Expertise Unit, Institut Pasteur, 75724 Paris, France., Laperche S; Institut national de la transfusion Sanguine (INTS), département d'études des Agents transmissibles par le sang (DATS), Centre national de référence risques infectieux transfusionnels, 75015 Paris, France., Covas DT; Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil., Kashima S; Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine [Transfus Clin Biol] 2019 Nov; Vol. 26 (4), pp. 234-239. Date of Electronic Publication: 2019 Jun 18.
DOI: 10.1016/j.tracli.2019.06.005
Abstrakt: Objectives: The objectives of this study were to evaluate the prevalence of Human Pegivirus-1 (HPgV-1) viremia and genotype diversity among healthy blood donors from the Eastern Brazilian Amazon (city of Macapá, State of Amapá). There is little information for prevalence and circulation of HPgV-1 in this remote Brazilian region.
Materials and Methods: We conducted a study evaluating the HPgV-1 RNA prevalence and circulating genotypes in 431 volunteer blood donors originating from the Eastern Brazilian Amazon. The obtained HPgV-1 positive samples were submitted to sequencing and genotyping analysis in order to examine the genotype diversity of this virus in the Brazilian Amazon.
Results: Our results demonstrated a prevalence of HPgV-1 RNA in 9.5% of the tested blood donors. The phylogenetic analyses of the detected positive samples showed the presence of HPgV-1 genotypes 1, 2 and 3. The most frequently detected genotype was 2 (78.0% of the cases) represented by sub-genotypes 2A (39.0%) and 2B (39.0%). At lower rates, genotypes 1 (14.6%) and 3 (7.4%) were also detected.
Conclusion: Our results revealed the presence of genotypes with European, Asiatic and African endemicity in Amazonian blood donors, probably due to the complex miscegenation processes that took place in this Brazilian region. More investigations, including information for the prevalence of HPgV-1 RNA in blood donors from other Latin American countries are needed to estimate the viremic rates and genotype distribution of this virus in a highly diverse continent like South America.
(Copyright © 2019 Société française de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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