Is the protein profile of pig Longissimus dorsi affected by gender and diet?

Autor: Paredi G; Interdepartmental Center for Safety, Technologies and Innovation in Agrifood (SITEIA.PARMA), University of Parma, Parma, Italy., Mori F; Interdepartmental Center for Safety, Technologies and Innovation in Agrifood (SITEIA.PARMA), University of Parma, Parma, Italy., de Marino MG; Interdepartmental Center for Safety, Technologies and Innovation in Agrifood (SITEIA.PARMA), University of Parma, Parma, Italy., Raboni S; Interdepartmental Center for Safety, Technologies and Innovation in Agrifood (SITEIA.PARMA), University of Parma, Parma, Italy; Institute of Biophysics, CNR, Pisa, Italy., Marchi L; Interdepartmental Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy., Galati S; Interdepartmental Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy., Buschini A; Interdepartmental Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy., Lo Fiego DP; Department of Life Sciences, University of Modena and Reggio-Emilia, Reggio Emilia, Italy; Interdepartmental Research Centre for Agri-Food Biological Resources Improvement and Valorization (BIOGEST-SITEIA), University of Modena and Reggio Emilia, P. le Europa, 1, I-42124 Reggio Emilia, Italy., Mozzarelli A; Interdepartmental Center for Safety, Technologies and Innovation in Agrifood (SITEIA.PARMA), University of Parma, Parma, Italy; Department of Food and Drug, University of Parma, Parma, Italy; Institute of Biophysics, CNR, Pisa, Italy. Electronic address: andrea.mozzarelli@unipr.it.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2019 Aug 30; Vol. 206, pp. 103437. Date of Electronic Publication: 2019 Jul 02.
DOI: 10.1016/j.jprot.2019.103437
Abstrakt: The impact of gender and diet on the proteome of Longissimus dorsi was addressed by 2D-PAGE analysis of male and female pigs, fed with a barley-based control diet and a diet enriched with extruded linseed and plant extracts. No statistically significant difference in protein number between female and male samples was found. Furthermore, PCA excluded gender-dependent protein clusters. For both the control and enriched diet, several spots exhibited at least a 1.5-fold intensity difference, but none showed a statistically relevant variation. Protein profiles PCA for both diets indicated that the first two principal components account up to 47% of total variance, with two diet-dependent separated clusters. Among 176 common spots, 29 exhibited >1.5 fold change, mostly more abundant in the control diet. PMF identified 14 distinct proteins, including myofibrillar proteins, glycolytic enzymes and myoglobin, thus suggesting a diet-dependent meat quality. A statistically significant increase in carbonylated proteins of enriched diet samples was detected using the 2,4-dinitrophenylhydrazine method but not using fluorescein-5-thiosemicarbazide-labeled bands. ROS induction and DNA oxidative damage, detected in a human cell line exposed to digested meat from both diets, further support the notion that the enriched diet does not protect against oxidative stress. SIGNIFICANCE: The comparison of the protein profile of female and male Longissimus dorsi from pigs fed by a control diet and a diet enriched with polyphenols, indicate no gender effect, whereas diet affects the abundance of several proteins, possibly linked to meat quality. Protein carbonylation was statistically higher in meat from the enriched diet, suggesting that polyphenols at the concentration present in the diet did not exert a protective effect against oxidation.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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