Comparison of methodologies for detecting Trypanosoma cruzi parasites by microscopic observation of microhematocrit capillary tubes.
| Autor: | Vera-Ku M; Laboratorio de Parasitología, Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi' Universidad Autónoma de Yucatán, Mérida, Yucatán, México.; Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México., Meza-González G; Laboratorio de Parasitología, Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi' Universidad Autónoma de Yucatán, Mérida, Yucatán, México.; Subdelegación Agropecuaria, Secretaria de Agricultura y Desarrollo Rural, Mexicali, Baja California Norte, México., Carlier Y; Laboratoire de Parasitologie, Faculté de Médecine, Université Libre de Bruxelles, Brussels, Belgium.; Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, New Orleans, Louisiana, United States of America., Truyens C; Laboratoire de Parasitologie, Faculté de Médecine, Université Libre de Bruxelles, Brussels, Belgium., Gamboa-León R; Laboratorio de Parasitología, Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi' Universidad Autónoma de Yucatán, Mérida, Yucatán, México.; Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, New Orleans, Louisiana, United States of America.; Laboratorio de Ciencias Biomédicas, Coordinación Académica Región Huasteca Sur, Universidad Autónoma de San Luis Potosí, Tamazunchale, San Luis Potosí, México. |
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| Jazyk: | angličtina |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2019 Jun 27; Vol. 52, pp. e20180505. Date of Electronic Publication: 2019 Jun 27. |
| DOI: | 10.1590/0037-8682-0505-2018 |
| Abstrakt: | Introduction: The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. Methods: The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. Results: The rotating method was easier, faster, and more sensitive than the others with 100% specificity. Conclusions: The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities. |
| Databáze: | MEDLINE |
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