The influence of temperature and pH on bacterial community composition of microbial mats in hot springs from Costa Rica.

Autor: Uribe-Lorío L; Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica., Brenes-Guillén L; Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica., Hernández-Ascencio W; Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica., Mora-Amador R; Escuela Centroamericana de Geología, Universidad de Costa Rica, San José, Costa Rica., González G; Escuela Centroamericana de Geología, Universidad de Costa Rica, San José, Costa Rica., Ramírez-Umaña CJ; Escuela Centroamericana de Geología, Universidad de Costa Rica, San José, Costa Rica., Díez B; Departamento de Microbiología y Genética, Pontificia Universidad Católica de Chile, Santiago, Chile., Pedrós-Alió C; Departamento de Biología de Sistemas, Centro Nacional de Biotecnología, CSIC, Madrid, Spain.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2019 Oct; Vol. 8 (10), pp. e893. Date of Electronic Publication: 2019 Jul 04.
DOI: 10.1002/mbo3.893
Abstrakt: We used the 16S rRNA gene pyrosequencing approach to investigate the microbial diversity and community composition in several Costa Rican hot springs alongside the latitudinal axis of the country, with a range of temperatures (37-63°C), pH (6-7.5) and other geochemical conditions. A principal component analyses of the physicochemical parameters showed the samples were separated into three geochemically distinct habitats associated with the location (North, Central, and South). Cyanobacteria and Chloroflexi comprised 93% of the classified community, the former being the most abundant phylum in all samples except for Rocas Calientes 1, (63°C, pH 6), where Chloroflexi and Deinococcus-Thermus represented 84% of the OTUs. Chloroflexi were more abundant as temperature increased. Proteobacteria, Bacteriodetes and Deinococcus-Thermus comprised 5% of the OTUs represented. Other Phyla were present in very small percentages (<1%). A LINKTREE analysis showed that the community structure of the mats was shaped primarily by pH, separating samples with pH > 6.6 from samples with pH < 6.4. Thus, both pH and temperature were relevant for community composition even within the moderate ranges of variables studied. These results provide a basis for an understanding of the physicochemical influences in moderately thermophilic microbial mats.
(© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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