Influence of antibiotics on biofilm formation by different clones of nosocomial Staphylococcus haemolyticus .

Autor: Pereira-Ribeiro PM; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Sued-Karam BR; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Faria YV; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Nogueira BA; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Colodette SS; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Fracalanzza SE; Universidade Federal do Rio de Janeiro (CCS/UFRJ), Rio de Janeiro, Brazil., Duarte JL; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Júnior RH; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil., Mattos-Guaraldi AL; Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil.
Jazyk: angličtina
Zdroj: Future microbiology [Future Microbiol] 2019 Jun; Vol. 14, pp. 789-799. Date of Electronic Publication: 2019 Jul 04.
DOI: 10.2217/fmb-2018-0230
Abstrakt: Staphylococcus haemolyticus is the most common organism among clinical isolatesof methicillin-resistant staphylococci. Aim: This study evaluated the ability to produce biofilm with the presence of the antibiotics (1/4 minimum inhibitory concentrations) of S. haemolyticus strains isolated from blood culture. Methods: Clonal distribution was assessed in pulsed-field gel electrophoresis. PCR assays were performed to detect mecA , icaA , aap , atlE , atl , fbp genes. S. haemolyticus strains grown in the presence of the antibiotics were investigated for biofilm formation on glass, polystyrene and catheter surfaces. Results: Biofilm formation was independent of the presence of the icaA and mecA genes, pulsed-field gel electrophoresis type. Vancomycin, oxacillin, moxifloxacin, rifampicin, teicoplanin, tigecycline and linezolid did not inhibit biofilm formation on abiotic surfaces. Conclusion: This study demonstrated that the biofilm formation process is complex and may not be related to ica gene carriage. Furthermore, in this study the biofilm formation was increased in the presence of antimicrobial agents.
Databáze: MEDLINE