| Autor: |
Burgos-Canul YY; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Canto-Canché B; Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Berezovski MV; Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada., Mironov G; Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada., Loyola-Vargas VM; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Barba de Rosa AP; IPICYT, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí, S.L.P., Mexico., Tzec-Simá M; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Brito-Argáez L; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Carrillo-Pech M; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Grijalva-Arango R; Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Muñoz-Pérez G; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico., Islas-Flores I; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico. islasign@cicy.mx. |
| Abstrakt: |
Pseudocercospora fijiensis causes black Sigatoka disease, the most important threat to banana. The cell wall is crucial for fungal biological processes, including pathogenesis. Here, we performed cell wall proteomics analyses of two P. fijiensis strains, the highly virulent Oz2b, and the less virulent C1233 strains. Strains were starved from nitrogen to mimic the host environment. Interestingly, in vitro cultures of the C1233 strain grew faster than Oz2b in PDB medium, suggesting that C1233 survives outside the host better than the highly virulent Oz2b strain. Both strains were submitted to nitrogen starvation and the cell wall proteins were isolated and subjected to nano-HPLC-MS/MS. A total of 2686 proteins were obtained from which only 240 had a known function and thus, bioinformatics analyses were performed on this group. We found that 90 cell wall proteins were shared by both strains, 21 were unique for Oz2b and 39 for C1233. Shared proteins comprised 24 pathogenicity factors, including Avr4 and Ecp6, two effectors from P. fijiensis, while the unique proteins comprised 16 virulence factors in C1233 and 11 in Oz2b. The P. fijiensis cell wall proteome comprised canonical proteins, but thirty percent were atypical, a feature which in other phytopathogens has been interpreted as contamination. However, a comparison with the identities of atypical proteins in other reports suggests that the P. fijiensis proteins we detected were not contaminants. This is the first proteomics analysis of the P. fijiensis cell wall and our results expands the understanding of the fundamental biology of fungal phytopathogens and will help to decipher the molecular mechanisms of pathogenesis and virulence in P. fijiensis. |
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