Methylome and transcriptome maps of human visceral and subcutaneous adipocytes reveal key epigenetic differences at developmental genes.

Autor:	Bradford ST; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia.; St Vincent's Clinical School, UNSW, Sydney, 2010, New South Wales, Australia., Nair SS; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; St Vincent's Clinical School, UNSW, Sydney, 2010, New South Wales, Australia., Statham AL; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., van Dijk SJ; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia., Peters TJ; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; St Vincent's Clinical School, UNSW, Sydney, 2010, New South Wales, Australia.; CSIRO Data61, North Ryde, 2113, New South Wales, Australia., Anwar F; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia., French HJ; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., von Martels JZH; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Sutcliffe B; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia., Maddugoda MP; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Peranec M; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Varinli H; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia.; Department of Biological Sciences, Macquarie University, North Ryde, 2019, New South Wales, Australia., Arnoldy R; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Buckley M; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; CSIRO Data61, North Ryde, 2113, New South Wales, Australia., Ross JP; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia., Zotenko E; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; St Vincent's Clinical School, UNSW, Sydney, 2010, New South Wales, Australia., Song JZ; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Stirzaker C; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; St Vincent's Clinical School, UNSW, Sydney, 2010, New South Wales, Australia., Bauer DC; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia., Qu W; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Swarbrick MM; Centre for Diabetes, Obesity and Endocrinology, The Westmead Institute, The University of Sydney, Westmead, 2145, New South Wales, Australia., Lutgers HL; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia.; Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands., Lord RV; St. Vincent's Centre for Applied Medical Research, Sydney, 2010, New South Wales, Australia., Samaras K; Department of Endocrinology, St Vincent's Hospital, Sydney, 2010, New South Wales, Australia.; Diabetes and Metabolism Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia., Molloy PL; CSIRO Health & Biosecurity, North Ryde, 2113, New South Wales, Australia. Peter.molloy@csiro.au., Clark SJ; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, 2010, New South Wales, Australia. s.clark@garvan.org.au.; St Vincent's Clinical School, UNSW, Sydney, 2010, New South Wales, Australia. s.clark@garvan.org.au.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2019 Jul 02; Vol. 9 (1), pp. 9511. Date of Electronic Publication: 2019 Jul 02.
DOI:	10.1038/s41598-019-45777-w
Abstrakt:	Adipocytes support key metabolic and endocrine functions of adipose tissue. Lipid is stored in two major classes of depots, namely visceral adipose (VA) and subcutaneous adipose (SA) depots. Increased visceral adiposity is associated with adverse health outcomes, whereas the impact of SA tissue is relatively metabolically benign. The precise molecular features associated with the functional differences between the adipose depots are still not well understood. Here, we characterised transcriptomes and methylomes of isolated adipocytes from matched SA and VA tissues of individuals with normal BMI to identify epigenetic differences and their contribution to cell type and depot-specific function. We found that DNA methylomes were notably distinct between different adipocyte depots and were associated with differential gene expression within pathways fundamental to adipocyte function. Most striking differential methylation was found at transcription factor and developmental genes. Our findings highlight the importance of developmental origins in the function of different fat depots.
Databáze:	MEDLINE
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