Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01.

Autor: Reis-Mansur MCPP; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Cardoso-Rurr JS; LaRBio - Radiations and Biology Laboratory, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Silva JVMA; Labio/Dimav/Inmetro - Laboratory of Tissue Bioengineering/Directorate of Metrology Applied to Life Sciences/National Institute of Metrology, Quality and Technology, Duque de Caxias, Brazil., de Souza GR; Research Institute for Natural Products, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Cardoso VDS; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Mansoldo FRP; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Pinheiro Y; LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Schultz J; LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Lopez Balottin LB; Labio/Dimav/Inmetro - Laboratory of Tissue Bioengineering/Directorate of Metrology Applied to Life Sciences/National Institute of Metrology, Quality and Technology, Duque de Caxias, Brazil., da Silva AJR; Research Institute for Natural Products, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Lage C; LaRBio - Radiations and Biology Laboratory, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Dos Santos EP; Faculty of Pharmacy, Galenico Development Laboratory, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Rosado AS; LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Vermelho AB; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. abvermelho@micro.ufrj.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Jul 02; Vol. 9 (1), pp. 9554. Date of Electronic Publication: 2019 Jul 02.
DOI: 10.1038/s41598-019-45840-6
Abstrakt: The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
Databáze: MEDLINE