Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus.

Autor: Postemsky PD; Laboratory of Biotechnology of Edible and Medicinal Mushrooms, Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS-UNS/CONICET), Camino de La Carrindaga Km7, 8000 Bahía Blanca, Buenos Aires, Argentina., Bidegain MA; Laboratory of Biotechnology of Edible and Medicinal Mushrooms, Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS-UNS/CONICET), Camino de La Carrindaga Km7, 8000 Bahía Blanca, Buenos Aires, Argentina., Lluberas G; Universidad de la República (UdelaR), Facultad de Ciencias, CIN, Mataojos s/n, Malvin Norte, Montevideo, 11200 Montevideo, Uruguay., Lopretti MI; Universidad de la República (UdelaR), Facultad de Ciencias, CIN, Mataojos s/n, Malvin Norte, Montevideo, 11200 Montevideo, Uruguay., Bonifacino S; Universidad de la República (UdelaR), Facultad de Ciencias, CIN, Mataojos s/n, Malvin Norte, Montevideo, 11200 Montevideo, Uruguay., Inés Landache M; Downstream Bioprocessing Laboratory, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany., Zygadlo JA; Instituto Multidisciplinario de Biología Vegetal, IMBIV-UNC/CONICET, Av. Vélez Sarsfield 1611, X5016GCA Córdoba, Argentina., Fernández-Lahore M; Downstream Bioprocessing Laboratory, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany., Omarini AB; Instituto de Ciencias de la Tierra y Ambientales de La Pampa, INCITAP-UNLPam/CONICET, Mendoza 109, L6300DUG Santa Rosa, La Pampa, Argentina. Electronic address: aomarini@yahoo.com.ar.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2019 Oct; Vol. 289, pp. 121692. Date of Electronic Publication: 2019 Jun 22.
DOI: 10.1016/j.biortech.2019.121692
Abstrakt: Pleurotus sapidus monokaryotic strains (Mk) were screened as a novel source of mycelia to valorize rice straw (RS), rice husks (RH) and sunflower seed hulls (SSH) into value-added products through solid-state fermentation (SSF). P. sapidus Dk3174 basidiospores were cultured in the presence of Remazol Brillant Blue R for strain selection, revealing the ligninolytic ability of emerging colonies. Further screening demonstrated the intraspecific variability in dye degradation and enzyme production of 63 strains. Growth rate, biomass and enzyme production in plates containing RS, RH or SSH pointed at MkP6 as a suitable strain for pilot-scale SSF. MkP6 presented a similar laccase profile as the parental Dk3174, being greater in pasteurized substrates (300-1200 U/Kg) than in sterilized substrates (30-250 U/Kg). Peroxidase represented 25% of the total ligninolytic activity measured. The SSH fermented biomass with MkP6 obtained good yields of nanocellulose (67%) and the saccharide release for ethanol production increased by 3-4 times.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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