| Autor: |
Gao S; Department of Anesthesiology, Weill Cornell Medical College, New York, NY, 10065, USA., Proekt A; Department of Anesthesiology, Weill Cornell Medical College, New York, NY, 10065, USA.; Laboratory of Neurobiology and Behavior, the Rockefeller University, New York, NY, 10065, USA., Renier N; ICM, Brain and Spine Institute, Hopital de la Pitie-Salpetriere, Sorbonne Universite, Inserm, CNRS, Paris, 75013, France., Calderon DP; Department of Anesthesiology, Weill Cornell Medical College, New York, NY, 10065, USA. dpc2003@med.cornell.edu.; Laboratory of Neurobiology and Behavior, the Rockefeller University, New York, NY, 10065, USA. dpc2003@med.cornell.edu., Pfaff DW; Laboratory of Neurobiology and Behavior, the Rockefeller University, New York, NY, 10065, USA. |
| Abstrakt: |
Multiple areas within the reticular activating system (RAS) can hasten awakening from sleep or light planes of anesthesia. However, stimulation in individual sites has shown limited recovery from deep global suppression of brain activity, such as coma. Here we identify a subset of RAS neurons within the anterior portion of nucleus gigantocellularis (aNGC) capable of producing a high degree of awakening represented by a broad high frequency cortical reactivation associated with organized movements and behavioral reactivity to the environment from two different models of deep pharmacologically-induced coma (PIC): isoflurane (1.25%-1.5%) and induced hypoglycemic coma. Activating aNGC neurons triggered awakening by recruiting cholinergic, noradrenergic, and glutamatergic arousal pathways. In summary, we identify an evolutionarily conserved population of RAS neurons, which broadly restore cerebral cortical activation and motor behavior in rodents through the coordinated activation of multiple arousal-promoting circuits. |
