Developing a pan-cancer research autopsy programme.

Autor:	Bavi P; Department of Pathology, University Health Network, Toronto, Ontario, Canada., Siva M; Department of Pathology, University Health Network, Toronto, Ontario, Canada., Abi-Saab T; Department of Pathology, University Health Network, Toronto, Ontario, Canada., Chadwick D; Department of Pathology, University Health Network, Toronto, Ontario, Canada., Dhani N; Department of Medical Oncology, Princess Margaret Cancer Centre, Toronto, Ontario, Canada., Butany J; Department of Pathology, University Health Network, Toronto, Ontario, Canada., Joshua AM; Department of Medical Oncology, Princess Margaret Cancer Centre, Toronto, Ontario, Canada roehrlm@mskcc.org anthony.joshua@doctor.com.; Kinghorn Cancer Centre, St. Vincent's Hospital, Sydney, New South Wales, Australia.; Garvan Institute of Medical Research, Sydney, New South Wales, Australia., Roehrl MH; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA roehrlm@mskcc.org anthony.joshua@doctor.com.; Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.; Weill Cornell Medicine, New York, New York, USA.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Jazyk:	angličtina
Zdroj:	Journal of clinical pathology [J Clin Pathol] 2019 Oct; Vol. 72 (10), pp. 689-695. Date of Electronic Publication: 2019 Jul 01.
DOI:	10.1136/jclinpath-2019-205874
Abstrakt:	Aims: Rapid procurement of a wide variety of metastatic and primary cancers and normal tissues after death through rapid autopsy opens largely unexplored avenues in cancer research. We describe a high-volume rapid research autopsy programme at a large academic medical centre. Methods: Advanced-stage cancer patients, most commonly inpatients in palliative care facilities, were approached to participate in a cancer research autopsy programme with the goal of acquiring multidimensionally annotated tissue for cancer research. On death of an enrolled patient, a predetermined notification plan was enacted, with the medical oncologist/clinical research coordinator informing a team of pathologists, researchers and allied staff. Quality assurance metrics were measured. Thereafter, tissues were annotated in a tissue bioinformatics database and linked to electronic patient records. All banked tissues were reviewed for tumour integrity, including DNA and RNA quality. Results: Over 100 rapid research autopsies from diverse cancer sites were performed, and specimens were procured and annotated with detailed clinical information, including treatment and response. Tissues were successfully enabling studies of tumour immunology, xenografts, genomics and proteomics. Conclusions: Large-scale rapid procurement and biobanking of cancer tissues from a rapid autopsy programme is feasible. Multidisciplinary integration between health and administrative staff from medical oncology, palliative care, pathology and biospecimen sciences is critical for the success of this challenging endeavour. Competing Interests: Competing interests: None declared. (© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Databáze:	MEDLINE
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