| Autor: |
Fahey JW; Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA. jfahey@jhmi.edu.; Division of Clinical Pharmacology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jfahey@jhmi.edu.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jfahey@jhmi.edu.; Center for Human Nutrition, Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA. jfahey@jhmi.edu., Wade KL; Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Stephenson KK; Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Panjwani AA; Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA.; Center for Human Nutrition, Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA., Liu H; Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Cornblatt G; Nutramax Laboratories Consumer Care, Inc., Edgewood, MD 21040, USA., Cornblatt BS; Nutramax Laboratories Consumer Care, Inc., Edgewood, MD 21040, USA., Ownby SL; Nutramax Laboratories Consumer Care, Inc., Edgewood, MD 21040, USA., Fuchs E; Division of Clinical Pharmacology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Holtzclaw WD; Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Cheskin LJ; Department of Health Behavior and Society, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205 USA. |
| Abstrakt: |
We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis. |
