PAS1-modified optical SIS sensor for highly sensitive and specific detection of toluene.

Autor: Dung TT; Bionanotechnology Research Center, 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, South Korea; KRIBB School, University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon, 34113, South Korea., Lee UJ; Bionanotechnology Research Center, 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, South Korea; Department of Biochemistry, College of Natural Sciences, Chungnam National University, 99 Daehangno, Yuseong-Gu, Daejeon, 34134, South Korea., Kim MH; Metabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, South Korea., Kim KE; Department of Biochemistry, College of Natural Sciences, Chungnam National University, 99 Daehangno, Yuseong-Gu, Daejeon, 34134, South Korea., Cho HM; KRIBB School, University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon, 34113, South Korea; Division of Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), 267 Gajeongno, Yuseong-Gu, Daejeon, 34113, South Korea., Fermin CD; Department of Biology, College of Arts & Sciences, Tuskegee University, 36088, USA; Ministerio de Salud Publica (MSP) & Caribbean Biotechnology Labs (CBL), Santo Domingo, DN, the Dominican Republic., Kim DH; Division of Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), 267 Gajeongno, Yuseong-Gu, Daejeon, 34113, South Korea. Electronic address: donghyung.kim@kriss.re.kr., Kim M; Bionanotechnology Research Center, 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, South Korea; KRIBB School, University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon, 34113, South Korea; Department of Biology, College of Arts & Sciences, Tuskegee University, 36088, USA. Electronic address: kimm@kribb.re.kr.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2019 Sep 15; Vol. 141, pp. 111469. Date of Electronic Publication: 2019 Jun 26.
DOI: 10.1016/j.bios.2019.111469
Abstrakt: We report on a novel solution immersed silicon (SIS) sensor modified with bio-receptor to detect toluene. To perform this approach, bio-receptor PAS1 which specifically interacts with toluene was chosen as a capture agent for SIS ellipsometric sensing. We constructed wild PAS1 and mutant PAS1 (F46A and F79Y) which are toluene binding-defective. Especially, we utilized an easily accessible capturing approach based on silica binding peptide (SBP) for direct immobilization of PAS1 on the SiO 2 surfaces. After the immobilization of SBP-tagged PAS1 to the sensing layers, PAS1-based SIS sensor was evaluated for its ability to recognize toluene. As a result, a significant up-shift in Psi (Ψ) was clearly observed with a low limit of detection (LOD) of 0.1 μM, when treated with toluene on wild PAS1-surface, but not on mutant PAS1-sensing layers, indicating the selective interactions between PAS1 and toluene molecule. The PAS1-SIS sensor showed no changes in Psi (Ψ), if any, negligible, when exposed to benzene, phenol, xylene and 4-nitrophenol as negative controls, thereby demonstrating the specificity of interaction between PAS1 and toluene. Taken together, our results strongly indicate that PAS1-modified ellipsometry sensor can provide a high fidelity system for the accurate and selective detection of toluene.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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