| Autor: |
Akgun FS; Department of Emergency Medicine, School of Medicine, Istanbul Maltepe University, Istanbul 34843, Turkey., Sirin DY; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag 59100, Turkey., Yilmaz I; Department of Medical Pharmacology, School of Medicine, Istanbul Medipol University, Istanbul 34810, Turkey., Karaarslan N; Department of Neurosurgery, School of Medicine, Namik Kemal University, Tekirdag 59100, Turkey., Ozbek H; Department of Medical Pharmacology, School of Medicine, Istanbul Medipol University, Istanbul 34810, Turkey., Simsek AT; Department of Neurosurgery, School of Medicine, Namik Kemal University, Tekirdag 59100, Turkey., Kaya YE; Department of Orthopedics and Traumatology, School of Medicine, Abant Izzet Baysal University, Bolu 14000, Turkey., Kaplan N; Department of Neurosurgery, Corlu Reyap Hospital, Istanbul Rumeli University, Tekirdag 59680, Turkey., Akyuva Y; Department of Neurosurgery, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul 34433, Turkey., Caliskan T; Department of Neurosurgery, School of Medicine, Namik Kemal University, Tekirdag 59100, Turkey., Ates O; Department of Neurosurgery, Istanbul Koc University Hospital, Istanbul Koc University, Istanbul 34010, Turkey. |
| Abstrakt: |
The present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells in vitro . After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone-treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin-1β and metalloproteinase (MMP)-19 genes were decreased, but MMP-13 and MMP-7 genes expressions were increased, as determined via reverse transcription-quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered. |
