Composition and ecology of bacterial and archaeal communities in anaerobic reactor fed with residual glycerol.

Autor: Vasconcelos EAF; Ecology and Natural Resources, Department of Biology, Federal University of Ceará, Campus Pici, Block 902, 60455-970, Fortaleza, CE, Brazil., Santaella ST; Institute of Marine Science, Federal University of Ceará, Av Abolição, 3207, 60165-081, Fortaleza, CE, Brazil., Viana MB; Institute of Marine Science, Federal University of Ceará, Av Abolição, 3207, 60165-081, Fortaleza, CE, Brazil., Dos Santos AB; Department of Hydraulics and Environmental Engineering, Federal University of Ceará, Campus Pici, Block 713, 60.451-970, Fortaleza, CE, Brazil., Pinheiro GC; Department of Technology and Environmental Management, Federal Institute of Education, Science and Technology of Ceará, Campus Maracanaú, Avenida Parque Central, Distrito Industrial I, Maracanaú, 61939-140, Brazil., Leitão RC; Embrapa Agroindústria Tropical, Rua Dra, Sara Mesquita, 2270, 60511-110, Fortaleza, CE, Brazil. Electronic address: renato.leitao@embrapa.br.
Jazyk: angličtina
Zdroj: Anaerobe [Anaerobe] 2019 Oct; Vol. 59, pp. 145-153. Date of Electronic Publication: 2019 Jun 27.
DOI: 10.1016/j.anaerobe.2019.06.014
Abstrakt: Glycerol, the main residue of biodiesel production, can be used to produce organic acids and energy through anaerobic digestion. This study aimed to assess microbial structure, diversity, productivity, and stability and the influence of these parameters on the performance of an anaerobic reactor. The experimental setup consisted of an upflow anaerobic sludge blanket (UASB) reactor fed residual glycerol and nutrients. The organic loading rate (OLR) was gradually increased through five stages, and sludge samples were collected at each, followed by DNA extraction and PCR denaturing gradient gel electrophoresis (PCR-DGGE). The resulting bands were excised, amplified, and purified. The results showed increased bacterial diversity and richness from the inoculum (Rr 38.72 and H 2.32) and along stages I and II, reaching the highest populational parameters (Rr 194.06 and H 3.32). The following stages promote decreases in richness and diversity, achieving the lowest populational parameters on this study (Rr 11.53 and H 2.04). Biogas production increased along with functional organization due to the specialization of the bacterial community and a decrease in the methanogenic population, both promoted by the increase in OLR.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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