Cutting Edge: CXCR3 Escapes X Chromosome Inactivation in T Cells during Infection: Potential Implications for Sex Differences in Immune Responses.

Autor:	Oghumu S; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210 oghumu.1@osu.edu abhay.satoskar@osumc.edu., Varikuti S; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210., Stock JC; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210., Volpedo G; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210., Saljoughian N; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210., Terrazas CA; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210., Satoskar AR; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210 oghumu.1@osu.edu abhay.satoskar@osumc.edu.
Jazyk:	angličtina
Zdroj:	Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2019 Aug 15; Vol. 203 (4), pp. 789-794. Date of Electronic Publication: 2019 Jun 28.
DOI:	10.4049/jimmunol.1800931
Abstrakt:	CXCR3, an X-linked gene, is subject to X chromosome inactivation (XCI), but it is unclear whether CXCR3 escapes XCI in immune cells. We determined whether CXCR3 escapes XCI in vivo, evaluated the contribution of allelic CXCR3 expression to the phenotypic properties of T cells during experimental infection with Leishmania, and examined the potential implications to sex differences in immune responses. We used a bicistronic CXCR3 dual-reporter mouse, with each CXCR3 allele linked to a green or red fluorescent reporter without affecting endogenous CXCR3 expression. Our results show that CXCR3 escapes XCI, biallelic CXCR3-expressing T cells produce more CXCR3 protein than monoallelic CXCR3-expressing cells, and biallelic CXCR3-expressing T cells produce more IFN-γ, IL-2, and CD69 compared with T cells that express CXCR3 from one allele during Leishmania mexicana infection. These results demonstrate that XCI escape by CXCR3 potentially contributes to the sex-associated bias observed during infection. (Copyright © 2019 by The American Association of Immunologists, Inc.)
Databáze:	MEDLINE
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