Assessment of a newly developed immunochromatographic assay for NDM-type metallo-β-lactamase producing Gram-negative pathogens in Myanmar.

Autor: Tada T; Department of Microbiology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan., Sekiguchi JI; Microbiology Research Division, Kohjin Bio, Co., Ltd. Chiyoda, Saitama, Japan., Watanabe S; Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan., Kuwahara-Arai K; Department of Microbiology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan., Mizutani N; Department of Microbiology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan., Yanagisawa I; Microbiology Research Division, Kohjin Bio, Co., Ltd. Chiyoda, Saitama, Japan., Hishinuma T; Department of Microbiology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan., Zan KN; National Health Laboratory, Yangon, Myanmar., Mya S; National Health Laboratory, Yangon, Myanmar., Tin HH; National Health Laboratory, Yangon, Myanmar., Kirikae T; Department of Microbiology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. tkirikae@ri.ncgm.go.jp.
Jazyk: angličtina
Zdroj: BMC infectious diseases [BMC Infect Dis] 2019 Jun 28; Vol. 19 (1), pp. 565. Date of Electronic Publication: 2019 Jun 28.
DOI: 10.1186/s12879-019-4147-4
Abstrakt: Background: To detect carbapenemase-producing Gram-negative bacteria in bacterial laboratories at medical settings, a new immunochromatographic assay for New Delhi metallo-β-lactamases (NDMs) was developed.
Methods: The immunochromatographic assay for New Delhi metallo-β-lactamases producers was developed using rat monoclonal antibodies against NDMs. The assessment was performed using 350 isolates of Gram-negative bacteria, including Acinetobacter baumannii (51 isolates), Enterobacteriaceae (163 isolates), and Pseudomonas aeruginosa (136 isolates) obtained from 2015 to 2017 in medical settings in Myanmar. Of them, 302 isolates were resistant to carbapenems, including imipenem and/or meropenem. The bla NDM genes were identified by PCR and sequencing.
Results: Of the 350 clinical isolates tested, 164 (46.9%) (60 isolates of Escherichia coli, 51 isolates of Klebsiella pneumoniae, 25 isolates of Enterobacter cloacae, 23 isolates of P. aeruginosa, and 5 isolates of A. baumannii) were positive on this assay, and all the positive isolates harbored genes encoding NDM-1, - 4, - 5 and - 7. The remaining 186 (53.1%) isolates negative on the assay did not harbor genes encoding NDMs. The assay had a specificity of 100% and a sensitivity of 100%. The assessment revealed that more than 90% of carbapenem-resistant Enterobacteriaceae produced NDMs.
Conclusions: The immunochromatographic assay is an easy-to-use and reliable kit for detection of NDMs-producing Gram-negative bacteria. The assay revealed that NDM-producing Enterobacteriaceae isolates are wide-spread in medical settings in Myanmar.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje