Expressed proteins and activated pathways in conditioned embryo culture media from IVF patients are diverse according to infertility factors.

Autor:	Bonetti TC; Disciplina de Ginecologia Endocrinológica, Departamento de Ginecologia, Escola Paulista de Medicina da Universidade Federal de São Paulo (UNIFESP-EPM). Brasil., Haddad DC; Setor Integrado de Reprodução Humana, Departamento de Urologia, Escola Paulista de Medicina da Universidade Federal de São Paulo (UNIFESP-EPM). Brasil., Domingues TS; Disciplina de Ginecologia Endocrinológica, Departamento de Ginecologia, Escola Paulista de Medicina da Universidade Federal de São Paulo (UNIFESP-EPM). Brasil.; Huntington - Medicina Reprodutiva. Brasil., Alegretti JR; Huntington - Medicina Reprodutiva. Brasil., Motta E; Disciplina de Ginecologia Endocrinológica, Departamento de Ginecologia, Escola Paulista de Medicina da Universidade Federal de São Paulo (UNIFESP-EPM). Brasil.; Huntington - Medicina Reprodutiva. Brasil., Seeley K; Proteomics and Mass Spectrometry Facility, Center for Drug Discovery and Innovation (CDDI), University of South Florida (USF). USA., Silva ID; Disciplina de Ginecologia Endocrinológica, Departamento de Ginecologia, Escola Paulista de Medicina da Universidade Federal de São Paulo (UNIFESP-EPM). Brasil.
Jazyk:	angličtina
Zdroj:	JBRA assisted reproduction [JBRA Assist Reprod] 2019 Oct 14; Vol. 23 (4), pp. 352-360. Date of Electronic Publication: 2019 Oct 14.
DOI:	10.5935/1518-0557.20190039
Abstrakt:	Objective: Given that the embryo culture medium secretome reflects the embryo development, we hypothesize that protein profiles are affected according to infertility factors, which can be responsible for detrimental embryonic developmental competence. The aim of this study was to screen the protein profile of conditioned embryo culture media in patients presenting deep infiltrating endometriosis (ENDO) and polycystic ovarian syndrome (PCOS) undergoing IVF, by proteomics approaches. The control group was constituted by tubal factor patients. Methods: Patients underwent in vitro fertilization (IVF) treatment as routine and oocytes were fertilized by ICSI. The embryos were group cultured until day 3 of development, and after transfer the culture media were collected. For the proteomics analysis, two pools of samples were prepared for groups CONTROL and PCOS, and 4 pools of samples for group DIE. Samples were prepared to deplete high abundant proteins and followed evaluated by high throughput proteomics approach. Results: The embryonic organ and tissue development were physiological functions activated, based on proteins identified in the 3 study groups of samples. The samples coming from DIE patients presented a high calcium activity and on the other hand, embryos coming from PCOS patients showed a decreased calcium action. Other pathways as grow factors through the EGF signaling pathway overexpressed in ENDO culture medium and protein kinase A in PCOS were also observed. Conclusions: Proteomic embryonic secretome will advance our knowledge of early embryogenesis and additionally could lead to improved selection of embryos for transfer warrants further investigation.
Databáze:	MEDLINE
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