| Autor: |
Rezig IM; Henry Wellcome Laboratory of Cell Biology, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Bremner SK; Henry Wellcome Laboratory of Cell Biology, Institute of Cardiovascular and Medical Studies, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Bhutta MS; Henry Wellcome Laboratory of Cell Biology, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Salt IP; Henry Wellcome Laboratory of Cell Biology, Institute of Cardiovascular and Medical Studies, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Gould GW; Henry Wellcome Laboratory of Cell Biology, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., McInerny CJ; Henry Wellcome Laboratory of Cell Biology, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. Chris.McInerny@glasgow.ac.uk.; Henry Wellcome Laboratory of Cell Biology, School of Life Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. Chris.McInerny@glasgow.ac.uk. |
| Abstrakt: |
The fission yeast Schizosaccharomyces pombe, an ascomycete fungus, is an established model organism for studying eukaryotic molecular and cellular events such as the cell cycle due to its powerful genetics, a sequenced genome, and the ease of molecular manipulation (Wood et al., Nature 415:871-880, 2002; Hoffman et al., Genetics 201:403-423, 2015). This chapter describes genetic and cytological methods to study endosomal sorting complex required for transport (ESCRT) function during the cell cycle in fission yeast. These include tetrad analysis to allow the creation of double mutants to test for genetic interactions by synthetic phenotype characterization, such as cellular growth and the analysis of division septa by calcofluor-white staining. |
