Autor: |
He L; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China., Li L; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China., Yu L; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China. ylc825@hotmail.com., He XZ; School of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North, New Zealand., Jiao R; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China., Xu C; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China., Zhang L; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China., Liu J; Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600, China. |
Abstrakt: |
Pyemotes zhonghuajia Yu, Zhang and He is a newly discovered native ectoparasitic mite that efficiently controls stem borers in China. To provide a steady and sufficient supply, extend adult lifespan and synchronize field augmentative releases of P. zhonghuajia, we determined the optimal cold storage temperature and duration by storing 1-day-old mated females at 8, 10 and 12 °C for 10-90 days with a 10-day interval in the laboratory. We then recorded mite survival during storage and monitored the post-storage reproductive performance of mites at a control temperature of 25 °C. We found that all mites survived at 10 and 12 °C for different durations, but mortality occurred when mites were stored at 8 °C for ≥ 30 days with more than 70% of mites dead when the storage duration prolonged up to 50 days. The proportion of reproductive females was higher at 10 °C but decreased with the prolonged storage duration at all test temperatures. Storage temperature had no significant effect on the pre-reproductive period and offspring sex ratio, whereas prolonged storage induced longer pre-reproductive period and lower proportion of female offspring. The reproductive period increased with increasing storage temperature and with prolonged storage up to 50 and 60 days; however, the longer reproductive period did not directly translate into greater reproductive output. We found that compared with the control, mites stored at 10 °C for up to 30 days did not significantly reduce their survival, proportion of reproductive success and number and sex ratio of offspring, suggesting that 10 °C and ≤ 30 days were the optimal cold storage temperature and duration, respectively, for post-mass production storage before the field augmentative release of P. zhonghuajia. |