The Serotonergic Raphe Promote Sleep in Zebrafish and Mice.

Autor:	Oikonomou G; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Altermatt M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Zhang RW; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Coughlin GM; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Montz C; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Gradinaru V; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: viviana@caltech.edu., Prober DA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: dprober@caltech.edu.
Jazyk:	angličtina
Zdroj:	Neuron [Neuron] 2019 Aug 21; Vol. 103 (4), pp. 686-701.e8. Date of Electronic Publication: 2019 Jun 24.
DOI:	10.1016/j.neuron.2019.05.038
Abstrakt:	The role of serotonin (5-HT) in sleep is controversial: early studies suggested a sleep-promoting role, but eventually the paradigm shifted toward a wake-promoting function for the serotonergic raphe. Here, we provide evidence from zebrafish and mice that the raphe are critical for the initiation and maintenance of sleep. In zebrafish, genetic ablation of 5-HT production by the raphe reduces sleep, sleep depth, and the homeostatic response to sleep deprivation. Pharmacological inhibition or ablation of the raphe reduces sleep, while optogenetic stimulation increases sleep. Similarly, in mice, ablation of the raphe increases wakefulness and impairs the homeostatic response to sleep deprivation, whereas tonic optogenetic stimulation at a rate similar to baseline activity induces sleep. Interestingly, burst optogenetic stimulation induces wakefulness in accordance with previously described burst activity of the raphe during arousing stimuli. These results indicate that the serotonergic system promotes sleep in both diurnal zebrafish and nocturnal rodents. VIDEO ABSTRACT. (Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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