One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia.

Autor: Barkham T; Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore., Zadoks RN; Institute of Biodiversity Animal Health and Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom., Azmai MNA; Department of Biology, Faculty of Science, and Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia., Baker S; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Bich VTN; Centre for Tropical Medicine, Oxford University Clinical Research Unit, Hanoi, Vietnam., Chalker V; Public Health England, Colindale, London, United Kingdom., Chau ML; Environmental Health Institute, National Environment Agency, Singapore.; National Centre for Food Science, Singapore Food Agency, Singapore., Dance D; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom., Deepak RN; Department of Laboratory Medicine, Khoo Teck Puat Hospital, Singapore., van Doorn HR; Oxford University Clinical Research Unit, Hanoi, Vietnam.; Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, United Kingdom., Gutierrez RA; Environmental Health Institute, National Environment Agency, Singapore.; National Centre for Infectious Diseases, Singapore., Holmes MA; Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom., Huong LNP; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Koh TH; Department of Microbiology, Singapore General Hospital, Singapore., Martins E; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal., Mehershahi K; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Newton P; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom., Ng LC; Environmental Health Institute, National Environment Agency, Singapore., Phuoc NN; Faculty of Fisheries, University of Agriculture and Forestry, Hue University, Hue City, Vietnam., Sangwichian O; Thailand Ministry of Public Health (MOPH)-US Centers for Disease Control and Prevention Collaboration (TUC), Nonthaburi, Thailand., Sawatwong P; Thailand Ministry of Public Health (MOPH)-US Centers for Disease Control and Prevention Collaboration (TUC), Nonthaburi, Thailand., Surin U; Nakhon Phanom General Hospital, Nakhon Phanom Provincial Health Office, Nakhon Phanom, Thailand., Tan TY; Department of Laboratory Medicine, Changi General Hospital, Singapore., Tang WY; Molecular Biology Laboratory, Tan Tock Seng Hospital, Singapore., Thuy NV; National Hospital for Obstetrics & Gynaecology, Hanoi, Vietnam., Turner P; Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, United Kingdom.; Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia., Vongsouvath M; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Lao People's Democratic Republic., Zhang D; Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, People's Republic of China., Whistler T; Thailand Ministry of Public Health (MOPH)-US Centers for Disease Control and Prevention Collaboration (TUC), Nonthaburi, Thailand.; Division of Global Health Protection, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America., Chen SL; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; Infectious Diseases Group, Genome Institute of Singapore, Singapore.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2019 Jun 27; Vol. 13 (6), pp. e0007421. Date of Electronic Publication: 2019 Jun 27 (Print Publication: 2019).
DOI: 10.1371/journal.pntd.0007421
Abstrakt: Background: In 2015, Singapore had the first and only reported foodborne outbreak of invasive disease caused by the group B Streptococcus (GBS; Streptococcus agalactiae). Disease, predominantly septic arthritis and meningitis, was associated with sequence type (ST)283, acquired from eating raw farmed freshwater fish. Although GBS sepsis is well-described in neonates and older adults with co-morbidities, this outbreak affected non-pregnant and younger adults with fewer co-morbidities, suggesting greater virulence. Before 2015 ST283 had only been reported from twenty humans in Hong Kong and two in France, and from one fish in Thailand. We hypothesised that ST283 was causing region-wide infection in Southeast Asia.
Methodology/principal Findings: We performed a literature review, whole genome sequencing on 145 GBS isolates collected from six Southeast Asian countries, and phylogenetic analysis on 7,468 GBS sequences including 227 variants of ST283 from humans and animals. Although almost absent outside Asia, ST283 was found in all invasive Asian collections analysed, from 1995 to 2017. It accounted for 29/38 (76%) human isolates in Lao PDR, 102/139 (73%) in Thailand, 4/13 (31%) in Vietnam, and 167/739 (23%) in Singapore. ST283 and its variants were found in 62/62 (100%) tilapia from 14 outbreak sites in Malaysia and Vietnam, in seven fish species in Singapore markets, and a diseased frog in China.
Conclusions: GBS ST283 is widespread in Southeast Asia, where it accounts for a large proportion of bacteraemic GBS, and causes disease and economic loss in aquaculture. If human ST283 is fishborne, as in the Singapore outbreak, then GBS sepsis in Thailand and Lao PDR is predominantly a foodborne disease. However, whether transmission is from aquaculture to humans, or vice versa, or involves an unidentified reservoir remains unknown. Creation of cross-border collaborations in human and animal health are needed to complete the epidemiological picture.
Competing Interests: I have read the journal's policy and the authors of this manuscript have the following competing interests: SLC and TB are named applicants on a patent for the ST83-specific PCR test used in this study.
Databáze: MEDLINE
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