Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique.

Autor: Pan N; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States., Standke SJ; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States., Kothapalli NR; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States., Sun M; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States., Bensen RC; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States., Burgett AWG; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States., Yang Z; Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2019 Jul 16; Vol. 91 (14), pp. 9018-9024. Date of Electronic Publication: 2019 Jun 27.
DOI: 10.1021/acs.analchem.9b01311
Abstrakt: Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using the Single-probe MS technique. The method was successfully used to perform rapid absolute quantifications of the anticancer drug irinotecan in individual mammalian cancer cells under ambient conditions in real time. Traditional liquid chromatography/mass spectrometry (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Single-probe qSCMS. This technique showcases heterogeneity of drug efficacy on the single-cell level.
Databáze: MEDLINE
Externí odkaz: