Expression profiling of immature florets of IR58025A, a wild-abortive cytoplasmic male sterile line of rice and its cognate, isonuclear maintainer line, IR58025B.

Autor: Pranathi K; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Kalyani MB; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Viraktamath BC; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Balachandran SM; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Hajira SK; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Koteshwar Rao P; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Kulakarni SR; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Rekha G; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Anila M; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Koushik MBVN; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Senguttuvel P; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Hariprasad AS; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Mangrautia SK; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Madhav MS; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India., Sundaram RM; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030 India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2019 Jul; Vol. 9 (7), pp. 278. Date of Electronic Publication: 2019 Jun 21.
DOI: 10.1007/s13205-019-1806-5
Abstrakt: Interaction between gene products encoded by the cytoplasm and nucleus form the core of wild abortive cytoplasmic male sterile (WA-CMS) system of hybrid breeding in rice. Gaining insights into such interactions can be helpful in the development of better three-line rice hybrids and also identify novel male sterility systems. In the present study, the whole transcriptome profiles of immature florets of IR58025A, a WA-CMS line and its isonuclear maintainer line, IR58025B, collected at pre-anthesis stage were compared to delineate the pathways involved in pollen abortion and male sterility. Among the 774 differentially expressed transcripts (DETs), 496 were down regulated and 278 were up regulated in IR58025A compared to IR58025B. The genes associated with oxidative stress response, defense response, etc. were significantly up-regulated, while those associated with respiration, cell wall modifications, pectinesterase activity, etc. were significantly down-regulated in the WA-CMS line. Gene ontology and pathway enrichment analyses revealed the down-regulation of both nuclear and organellar genes involved in key metabolic processes of cell respiration, photosynthesis and other energy yielding metabolites in IR58025A, relative to IR58025B, indicating a general shift toward conservation of energy and other key resources in the florets of WA-CMS line. The data derived from RNA-Seq analysis were validated through qRT-PCR analysis. Based on the results obtained, it can be hypothesized that pollen abortion principally occurs due to up-regulation of pathways leading to oxidative stress leading to energy starvation conditions in consonance with reduced expression of genes associated with the cell wall formation, respiration, and other key metabolic processes.
Competing Interests: Conflict of interestOn behalf of all authors, the corresponding author states that there is no conflict of interest.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje