| Autor: |
Soe TW; Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa-ku, Nagoya 464-8601 , Japan.; Department of Chemistry , University of Yangon , Yangon 11041 , Myanmar., Han C; Research Center for Materials Science , Nagoya University , Chikusa-ku, Nagoya 464-8602 , Japan., Fudou R; R&D Planning Department , Ajinomoto Co., Inc. , Chuo-ku, Tokyo 104-8315 , Japan., Kaida K; Institute of Life Science , Ajinomoto Co., Inc. , Kawasaki , Kanagawa 210-8681 , Japan., Sawaki Y; Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa-ku, Nagoya 464-8601 , Japan., Tomura T; Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa-ku, Nagoya 464-8601 , Japan., Ojika M; Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa-ku, Nagoya 464-8601 , Japan. |
| Abstrakt: |
Seven new cyclic depsipeptides, clavariopsins C-I ( 3 - 9 ), together with two known congeners, clavariopsins A and B ( 1 and 2 ), were isolated from the aquatic hyphomycete Clavariopsis aquatica . Their planar structures, which consist of nine amino acids and one α-hydroxy acid, were elucidated by NMR spectroscopy and HRESIMS. The absolute configurations were established by the advanced Marfey's method and chiral-phase HPLC analysis. Their antifungal and cytotoxic activities were evaluated against six plant pathogenic fungi ( Botrytis cinerea , Magnaporthe oryzae , Colletotrichum orbiculare , Fusarium oxysporum , Alternaria alternata , and Aspergillus niger ) and a cancer cell line (HeLa-S3), respectively. The majority of the compounds exhibited potent antifungal activity against the fungi tested (minimum inhibition dose = 0.01-10 μg/disk) and induced hyphal swelling in A. niger (minimum effective dose = 0.3-3 μg/disk), whereas the compounds exhibited no cytotoxicity toward the cancer cell line. The results suggest that the clavariopsins could be a promising class of antifungal agents. |
