Microtubule glycylation promotes attachment of basal bodies to the cell cortex.

Autor:	Junker AD; Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Soh AWJ; Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA., O'Toole ET; Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80302, USA., Meehl JB; Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80302, USA., Guha M; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA., Winey M; Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA., Honts JE; Department of Biology, Drake University, 2507 University Avenue, Des Moines, IA 50311, USA., Gaertig J; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA., Pearson CG; Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA Chad.Pearson@ucdenver.edu.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2019 Aug 07; Vol. 132 (15). Date of Electronic Publication: 2019 Aug 07.
DOI:	10.1242/jcs.233726
Abstrakt:	Motile cilia generate directed hydrodynamic flow that is important for the motility of cells and extracellular fluids. To optimize directed hydrodynamic flow, motile cilia are organized and oriented into a polarized array. Basal bodies (BBs) nucleate and position motile cilia at the cell cortex. Cytoplasmic BB-associated microtubules are conserved structures that extend from BBs. By using the ciliate, Tetrahymena thermophila , combined with EM-tomography and light microscopy, we show that BB-appendage microtubules assemble coincidently with new BB assembly and that they are attached to the cell cortex. These BB-appendage microtubules are specifically marked by post translational modifications of tubulin, including glycylation. Mutations that prevent glycylation shorten BB-appendage microtubules and disrupt BB positioning and cortical attachment. Consistent with the attachment of BB-appendage microtubules to the cell cortex to position BBs, mutations that disrupt the cellular cortical cytoskeleton disrupt the cortical attachment and positioning of BBs. In summary, BB-appendage microtubules promote the organization of ciliary arrays through attachment to the cell cortex. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2019. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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