The NuRD chromatin-remodeling complex enzyme CHD4 prevents hypoxia-induced endothelial Ripk3 transcription and murine embryonic vascular rupture.

Autor: Colijn S; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73190, USA., Gao S; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73190, USA., Ingram KG; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73190, USA.; Stowers Institute for Medical Research, Kansas City, MO, 64110, USA., Menendez M; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA., Muthukumar V; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; The Jackson Laboratory, Bar Harbor, ME, 04609, USA., Silasi-Mansat R; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA., Chmielewska JJ; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Centre of New Technologies, University of Warsaw, 02-097, Warsaw, Poland., Hinsdale M; Department of Physiological Sciences, Oklahoma State University, Stillwater, OK, 74078, USA., Lupu F; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73190, USA., Griffin CT; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA. courtney-griffin@omrf.org.; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73190, USA. courtney-griffin@omrf.org.
Jazyk: angličtina
Zdroj: Cell death and differentiation [Cell Death Differ] 2020 Feb; Vol. 27 (2), pp. 618-631. Date of Electronic Publication: 2019 Jun 24.
DOI: 10.1038/s41418-019-0376-8
Abstrakt: Physiological hypoxia can trigger transcriptional events that influence many developmental processes during mammalian embryogenesis. One way that hypoxia affects transcription is by engaging chromatin-remodeling complexes. We now report that chromodomain helicase DNA binding protein 4 (CHD4), an enzyme belonging to the nucleosome remodeling and deacetylase (NuRD) chromatin-remodeling complex, is required for transcriptional repression of the receptor-interacting protein kinase 3 (Ripk3)-a critical executor of the necroptosis cell death program-in hypoxic murine embryonic endothelial cells. Genetic deletion of Chd4 in murine embryonic endothelial cells in vivo results in upregulation of Ripk3 transcripts and protein prior to vascular rupture and lethality at midgestation, and concomitant deletion of Ripk3 partially rescues these phenotypes. In addition, CHD4 binds to and prevents acetylation of the Ripk3 promoter in cultured endothelial cells grown under hypoxic conditions to prevent excessive Ripk3 transcription. These data demonstrate that excessive RIPK3 is detrimental to embryonic vascular integrity and indicate that CHD4 suppresses Ripk3 transcription when the embryonic environment is particularly hypoxic prior to the establishment of fetal-placental circulation at midgestation. Altogether, this research provides new insights into regulators of Ripk3 transcription and encourages future studies into the mechanism by which excessive RIPK3 damages embryonic blood vessels.
Databáze: MEDLINE
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