Features of diclofenac biodegradation by Rhodococcus ruber IEGM 346.

Autor: Ivshina IB; Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia. ivshina@iegm.ru.; Perm State National Research University, 15 Bukirev Street, 614990, Perm, Russia. ivshina@iegm.ru., Tyumina EA; Perm State National Research University, 15 Bukirev Street, 614990, Perm, Russia., Kuzmina MV; Perm State Pharmaceutical Academy, 2 Polevaya Street, 614990, Perm, Russia., Vikhareva EV; Perm State Pharmaceutical Academy, 2 Polevaya Street, 614990, Perm, Russia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Jun 24; Vol. 9 (1), pp. 9159. Date of Electronic Publication: 2019 Jun 24.
DOI: 10.1038/s41598-019-45732-9
Abstrakt: This study investigated the ability of rhodococci to biodegrade diclofenac (DCF), one of the polycyclic non-steroidal anti-inflammatory drugs (NSAIDs) most frequently detected in the environment. Rhodococcus ruber strain IEGM 346 capable of complete DCF biodegradation (50 µg/L) over 6 days was selected. It is distinguished by the ability to degrade DCF at high (50 mg/L) concentrations unlike other known biodegraders. The DCF decomposition process was accelerated by adding glucose and due to short-term cell adaptation to 5 µg/L DCF. The most typical responses to DCF exposure observed were the changed ζ-potential of bacterial cells; increased cell hydrophobicity and total cell lipid content; multi-cellular conglomerates formed; and the changed surface-to-volume ratio. The obtained findings are considered as mechanisms of rhodococcal adaptation and hence their increased resistance to toxic effects of this pharmaceutical pollutant. The proposed pathways of bacterial DCF metabolisation were described. The data confirming the C-N bond cleavage and aromatic ring opening in the DCF structure were obtained.
Databáze: MEDLINE
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