An unusual and vital protein with guanylate cyclase and P4-ATPase domains in a pathogenic protist.

Autor: Günay-Esiyok Ö; Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany., Scheib U; Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany., Noll M; Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany., Gupta N; Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany Gupta.Nishith@hu-berlin.de.
Jazyk: angličtina
Zdroj: Life science alliance [Life Sci Alliance] 2019 Jun 24; Vol. 2 (3). Date of Electronic Publication: 2019 Jun 24 (Print Publication: 2019).
DOI: 10.26508/lsa.201900402
Abstrakt: cGMP signaling is one of the master regulators of diverse functions in eukaryotes; however, its architecture and functioning in protozoans remain poorly understood. Herein, we report an exclusive guanylate cyclase coupled with N-terminal P4-ATPase in a common parasitic protist, Toxoplasma gondii This bulky protein (477-kD), termed Tg ATPase P -GC to fairly reflect its envisaged multifunctionality, localizes in the plasma membrane at the apical pole of the parasite, whereas the corresponding cGMP-dependent protein kinase ( Tg PKG) is distributed in the cytomembranes. Tg ATPase P -GC is refractory to genetic deletion, and its CRISPR/Cas9-assisted disruption aborts the lytic cycle of T. gondii Besides, Cre/loxP-mediated knockdown of Tg ATPase P -GC reduced the synthesis of cGMP and inhibited the parasite growth due to impairments in the motility-dependent egress and invasion events. Equally, repression of Tg PKG by a similar strategy recapitulated phenotypes of the Tg ATPase P -GC-depleted mutant. Notably, despite a temporally restricted function, Tg ATPase P -GC is expressed constitutively throughout the lytic cycle, entailing a post-translational regulation of cGMP signaling. Not least, the occurrence of Tg ATPase P -GC orthologs in several other alveolates implies a divergent functional repurposing of cGMP signaling in protozoans, and offers an excellent drug target against the parasitic protists.
(© 2019 Günay-Esiyok et al.)
Databáze: MEDLINE
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