Human mastadenovirus in water, sediment, sea surface microlayer, and bivalve mollusk from southern Brazilian beaches.

Autor: Gularte JS; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil. Electronic address: 0159014@feevale.br., Girardi V; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., Demoliner M; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., de Souza FG; Laboratório de Vírus, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, no 6627, Belo Horizonte, MG, Brazil., Filippi M; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., Eisen AKA; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., Mena KD; School of Public Health, The University of Texas Health Science Center at Houston, El Paso, TX 79902, USA., de Quevedo DM; Programa de Pós-Graduação em Qualidade Ambiental, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., Rigotto C; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., de Barros MP; Curso de Ciências Biológicas, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil., Spilki FR; Laboratório de Microbiologia Molecular, Universidade Feevale, ERS 239, no 2755, Novo Hamburgo, RS 93352-000, Brazil.
Jazyk: angličtina
Zdroj: Marine pollution bulletin [Mar Pollut Bull] 2019 May; Vol. 142, pp. 335-349. Date of Electronic Publication: 2019 Apr 03.
DOI: 10.1016/j.marpolbul.2018.12.046
Abstrakt: Anthropogenic contamination of beaches in the south of Brazil was assessed by detection of Escherichia coli, human mastadenovirus species C (HAdV-C) and F (HAdV-F) and hepatitis E virus (HEV). Sampling was carried out in October (2016), and in January, April and July (2017). Water, sediment, sea surface microlayer (SML), bivalves, and air sentinel samples were evaluated. Quantitative microbiological risk assessment (QMRA) was used to estimate the probability of swimmer infection. HAdV-C was present in 26% of the samples, for both qPCR and viral isolation. The highest rates of detection in genomic copies (GC) were in water (2.42E+10 GC/L), SML (2.08E+10 GC/L), sediment (3.82E+08 GC/g) and bivalves (3.91E+07 GC/g). QMRA estimated daily and annual risks with a maximum value (9.99E-01) in almost all of the samples. Viable HAdV-C was often detected in the SML, pointing that this is a source of infection for people bathing in these waters.
(Copyright © 2018. Published by Elsevier Ltd.)
Databáze: MEDLINE
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