Contractile dysfunction and nitrergic dysregulation in small intestine of a primate model of Parkinson's disease.

Autor:	Coletto E; 1Neurodegenerative Disease Research Group, Institute of Pharmaceutical Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK., Dolan JS; 2Department of Clinical and Pharmaceutical Sciences, University of Hertfordshire, Hatfield, UK., Pritchard S; 2Department of Clinical and Pharmaceutical Sciences, University of Hertfordshire, Hatfield, UK., Gant A; 2Department of Clinical and Pharmaceutical Sciences, University of Hertfordshire, Hatfield, UK., Hikima A; 1Neurodegenerative Disease Research Group, Institute of Pharmaceutical Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK., Jackson MJ; 1Neurodegenerative Disease Research Group, Institute of Pharmaceutical Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK., Benham CD; 2Department of Clinical and Pharmaceutical Sciences, University of Hertfordshire, Hatfield, UK., Chaudhuri KR; 3Basic and Clinical Neuroscience, The Maurice Wohl Clinical Neuroscience Institute, Kings College London, London, UK., Rose S; 1Neurodegenerative Disease Research Group, Institute of Pharmaceutical Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK., Jenner P; 1Neurodegenerative Disease Research Group, Institute of Pharmaceutical Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK., Iravani MM; 2Department of Clinical and Pharmaceutical Sciences, University of Hertfordshire, Hatfield, UK.
Jazyk:	angličtina
Zdroj:	NPJ Parkinson's disease [NPJ Parkinsons Dis] 2019 Jun 10; Vol. 5, pp. 10. Date of Electronic Publication: 2019 Jun 10 (Print Publication: 2019).
DOI:	10.1038/s41531-019-0081-9
Abstrakt:	Bowel dysfunction is a common non-motor symptom in Parkinson's disease (PD). The main contractile neurotransmitter in the GI tract is acetylcholine (ACh), while nitric oxide (NO) causes the relaxation of smooth muscle in addition to modulating ACh release. The aim of this study was to characterise functional and neurochemical changes in the isolated ileum of the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated marmoset, an established model of PD motor dysfunction. While NO-synthase inhibitor L-NAME concentration dependently augmented the neurogenically-evoked contractions and inhibited the relaxations in normal tissues, it had no effects on the MPTP ileum. Immunohistochemical analyses of the myenteric plexus showed that ChAT-immunoreactivity (-ir) was significantly reduced and the density of the enteric glial cells as shown by SOX-10-ir was increased. However, no change in TH-, 5-HT-, VIP- or nNOS-ir was observed in the MPTP tissues. The enhancement of the neurogenically-evoked contractions and the inhibition of the relaxation phase by L-NAME in the control tissues is in line with NO's direct relaxing effect on smooth muscle and its indirect inhibitory effect on ACh release. The absence of the relaxation and the inefficacy of L-NAME in the MPTP tissues suggests that central dopaminergic loss dopamine may eventually lead to the impairment of NO signal coupling that affects bowel function, and this may be the result of a complex dysregulation at the level of the neuroeffector junction. Competing Interests: Competing interestsThe authors declare no competing interests.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu