| Autor: |
Govindasamy N; Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Münster, Germany., Bedzhov I; Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Münster, Germany. ivan.bedzhov@mpi-muenster.mpg.de. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 2006, pp. 373-382. |
| DOI: |
10.1007/978-1-4939-9566-0_25 |
| Abstrakt: |
Key developmental processes of cell fate decisions and morphogenetic transformations take place during the periimplantation and early postimplantation stages of mouse embryogenesis. However analysing these fundamental events relies on direct observations of cultured embryos, which are challenging to obtain. To address this challenge, here we provide a detailed protocol describing a workflow for isolating early implanted embryos, removing of redundant extraembryonic tissues and describing the culture conditions that support further embryo development in vitro. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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