Monitoring biomolecule concentrations in tissue using a wearable droplet microfluidic-based sensor.

Autor:	Nightingale AM; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, UK., Leong CL; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, UK.; Department of Bioengineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Burnish RA; Critical Care/ Anaesthesia and Perioperative Medicine Research Unit, University Hospital Southampton NHS Foundation Trust, Tremona Road, Southampton, SO16 6YD, UK., Hassan SU; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, UK., Zhang Y; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, UK., Clough GF; Faculty of Medicine, University of Southampton, Southampton, SO17 1BJ, UK., Boutelle MG; Department of Bioengineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Voegeli D; Faculty of Environmental and Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK.; Now at Department of Sport, Exercise & Health, University of Winchester, Winchester, SO22 4NR, UK., Niu X; Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, UK. x.niu@soton.ac.uk.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2019 Jun 21; Vol. 10 (1), pp. 2741. Date of Electronic Publication: 2019 Jun 21.
DOI:	10.1038/s41467-019-10401-y
Abstrakt:	Knowing how biomarker levels vary within biological fluids over time can produce valuable insight into tissue physiology and pathology, and could inform personalised clinical treatment. We describe here a wearable sensor for monitoring biomolecule levels that combines continuous fluid sampling with in situ analysis using wet-chemical assays (with the specific assay interchangeable depending on the target biomolecule). The microfluidic device employs a droplet flow regime to maximise the temporal response of the device, using a screw-driven push-pull peristaltic micropump to robustly produce nanolitre-sized droplets. The fully integrated sensor is contained within a small (palm-sized) footprint, is fully autonomous, and features high measurement frequency (a measurement every few seconds) meaning deviations from steady-state levels are quickly detected. We demonstrate how the sensor can track perturbed glucose and lactate levels in dermal tissue with results in close agreement with standard off-line analysis and consistent with changes in peripheral blood levels.
Databáze:	MEDLINE
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