FishNET: An automated relational database for zebrafish colony management.

Autor: Cantu Gutierrez A; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas, United States of America.; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America., Cantu Gutierrez M; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas, United States of America.; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.; Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America., Rhyner AM; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas, United States of America.; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America., Ruiz OE; Department of Genetics, MD Anderson Cancer Center, Houston, Texas, United States of America., Eisenhoffer GT; Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America.; Department of Genetics, MD Anderson Cancer Center, Houston, Texas, United States of America., Wythe JD; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas, United States of America.; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.; Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2019 Jun 20; Vol. 17 (6), pp. e3000343. Date of Electronic Publication: 2019 Jun 20 (Print Publication: 2019).
DOI: 10.1371/journal.pbio.3000343
Abstrakt: The zebrafish Danio rerio is a powerful model system to study the genetics of development and disease. However, maintenance of zebrafish husbandry records is both time intensive and laborious, and a standardized way to manage and track the large amount of unique lines in a given laboratory or centralized facility has not been embraced by the field. Here, we present FishNET, an intuitive, open-source, relational database for managing data and information related to zebrafish husbandry and maintenance. By creating a "virtual facility," FishNET enables users to remotely inspect the rooms, racks, tanks, and lines within a given facility. Importantly, FishNET scales from one laboratory to an entire facility with several laboratories to multiple facilities, generating a cohesive laboratory and community-based platform. Automated data entry eliminates confusion regarding line nomenclature and streamlines maintenance of individual lines, while flexible query forms allow researchers to retrieve database records based on user-defined criteria. FishNET also links associated embryonic and adult biological samples with data, such as genotyping results or confocal images, to enable robust and efficient colony management and storage of laboratory information. A shared calendar function with email notifications and automated reminders for line turnover, automated tank counts, and census reports promote communication with both end users and administrators. The expected benefits of FishNET are improved vivaria efficiency, increased quality control for experimental numbers, and flexible data reporting and retrieval. FishNET's easy, intuitive record management and open-source, end-user-modifiable architecture provides an efficient solution to real-time zebrafish colony management for users throughout a facility and institution and, in some cases, across entire research hubs.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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