Effects of Carpobrotus edulis Extract on Oxidative Stress and 158N Oligodendrocyte Death.

Autor:	Zarrouk A; Laboratory of Biochemistry, Faculty of Medicine Sousse, 4002, Sousse, University of Sousse, Tunisia; Laboratory UR12ES05 'Nutrition, Fonctionnels Food & Vascular Health', Faculty of Medicine Monastir, 5019 University of Monastir, Tunisia., Smach MA; Laboratory of Biochemistry, Faculty of Medicine Sousse, 4002, Sousse, University of Sousse, Tunisia., Hafsa J; Laboratory of Biochemistry, Faculty of Medicine Sousse, 4002, Sousse, University of Sousse, Tunisia., Sghaier R; Laboratory UR12ES05 'Nutrition, Fonctionnels Food & Vascular Health', Faculty of Medicine Monastir, 5019 University of Monastir, Tunisia., Majdoub H; Laboratory of Interfaces and Advanced Materials, Faculty of Sciences of Monastir, 5000 University of Monastir, Tunisie., Hammami M; Laboratory UR12ES05 'Nutrition, Fonctionnels Food & Vascular Health', Faculty of Medicine Monastir, 5019 University of Monastir, Tunisia., Charfeddine B; Laboratory of Biochemistry, Faculty of Medicine Sousse, 4002, Sousse, University of Sousse, Tunisia.
Jazyk:	angličtina
Zdroj:	Biomedical and environmental sciences : BES [Biomed Environ Sci] 2019 Apr; Vol. 32 (4), pp. 291-299.
DOI:	10.3967/bes2019.039
Abstrakt:	Objective: Age-related diseases, including neurodegenerative diseases, are associated with oxidative stress and lipid peroxidation, and increase the levels of cholesterol auto-oxidation products such as 7β-hydroxycholesterol (7β-OHC). Thus, it is imperative to identify agents that can prevent 7β-OHC-induced side-effects. Methods: We evaluated the potential protective effects of Carpobrotus edulis ethanol-water extract (EWe) on murine oligodendrocytes (158N) cultured in the absence or presence of 7β-OHC (20 μg/mL, 24 h). The cells were incubated with EWe (20-200 µg/mL) 2 h before 7β-OHC treatment. Mitochondrial activity and cell growth were evaluated with the MTT assay. Photometric methods were used to analyze antioxidant enzyme [catalase (CAT) and glutathione peroxidase (GPx)] activities and the generation of lipid and protein oxidation products [malondialdehyde (MDA), conjugated diene (CD), and carbonylated proteins (CPs)]. Results: Treatment with 7β-OHC induced cell death and oxidative stress (reflected by alteration in CAT and SOD activities). Overproduction of lipid peroxidation products (MDA and CDs) and CPs was also reported. The cytotoxic effects associated with 7β-OHC were attenuated by 160 μg/mL of EWe of C. edulis. Cell death induced by 7β-OHC treatment was ameliorated, GPx and CAT activities were restored to normal, and MDA, CD, and CP levels were reduced following C. edulis extract treatment. Conclusion: These data demonstrate the protective activities of C. edulis EWe against 7β-OHC-induced disequilibrium in the redox status of 158N cells, indicative of the potential role of this plant extract in the prevention of neurodegenerative diseases. (Copyright © 2019 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)
Databáze:	MEDLINE
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