Label-Free Multiphoton Microscopy: The Origin of Fluorophores and Capabilities for Analyzing Biochemical Processes.

Autor: Shirshin EA; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia. shirshin@lid.phys.msu.ru.; Institute of Spectroscopy, Russian Academy of Sciences, Troitsk, 108840, Moscow, Russia., Yakimov BP; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia., Darvin ME; Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, 10117, Germany., Omelyanenko NP; N. N. Priorov National Medical Research Center of Traumatology and Orthopaedics, Moscow, 127299, Russia., Rodionov SA; N. N. Priorov National Medical Research Center of Traumatology and Orthopaedics, Moscow, 127299, Russia., Gurfinkel YI; Medical Scientific-Educational Center of Lomonosov Moscow State University, Moscow, 119192, Russia., Lademann J; Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, 10117, Germany., Fadeev VV; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia., Priezzhev AV; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia.
Jazyk: angličtina
Zdroj: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2019 Jan; Vol. 84 (Suppl 1), pp. S69-S88.
DOI: 10.1134/S0006297919140050
Abstrakt: Multiphoton microscopy (MPM) is a method of molecular imaging and specifically of intravital imaging that is characterized by high spatial resolution in combination with a greater depth of penetration into the tissue. MPM is a multimodal method based on detection of nonlinear optical signals - multiphoton fluorescence and optical harmonics - and also allows imaging with the use of the parameters of fluorescence decay kinetics. This review describes and discusses photophysical processes within major reporter molecules used in MPM with endogenous contrasts and summarizes several modern experiments that illustrate the capabilities of label-free MPM for molecular imaging of biochemical processes in connective tissue and cells.
Databáze: MEDLINE