| Autor: |
Cepas V; ISGlobal, Hospital Clínic-Universitat de Barcelona, 08036 Barcelona, Spain. virginio.cepas@isglobal.org., López Y; ISGlobal, Hospital Clínic-Universitat de Barcelona, 08036 Barcelona, Spain. yuly.lopez@isglobal.org., Gabasa Y; ISGlobal, Hospital Clínic-Universitat de Barcelona, 08036 Barcelona, Spain. gabasagyaiza@gmail.com., Martins CB; Coimbra Collection of Algae (ACOI), Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal. martinscsb@gmail.com., Ferreira JD; Coimbra Collection of Algae (ACOI), Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal. ferreirajoanadias@gmail.com., Correia MJ; Coimbra Collection of Algae (ACOI), Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal. mariajacorreia1@gmail.com., Santos LMA; Coimbra Collection of Algae (ACOI), Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal. liliamas@ci.uc.pt., Oliveira F; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), 4450-208 Porto, Portugal. up201510053@fc.up.pt., Ramos V; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), 4450-208 Porto, Portugal. vtr.rms@gmail.com., Reis M; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), 4450-208 Porto, Portugal. mariana.a.reis@gmail.com., Castelo-Branco R; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), 4450-208 Porto, Portugal. raquel.castelobranco.12@gmail.com., Morais J; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), 4450-208 Porto, Portugal. joaopmorais@gmail.com., Vasconcelos V; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), 4450-208 Porto, Portugal. vmvascon@fc.up.pt.; Faculty of Sciences, University of Porto, 4450-208 Porto, Portugal. vmvascon@fc.up.pt., Probert I; Roscoff Culture Collection, Sorbonne University/CNRS, Roscoff Biological Station, 29680 Roscoff, France. probert@sb-roscoff.fr., Guilloud E; Roscoff Culture Collection, Sorbonne University/CNRS, Roscoff Biological Station, 29680 Roscoff, France. emilie.guilloud@gmail.com., Mehiri M; Marine Natural Products Team, Nice Institute of Chemistry, UMR 7272 University Nice Côte d'Azur/CNRS, 60103 Nice, France. Mohamed.MEHIRI@unice.fr., Soto SM; ISGlobal, Hospital Clínic-Universitat de Barcelona, 08036 Barcelona, Spain. sara.soto@isglobal.org. |
| Abstrakt: |
Bacterial biofilms are complex biological systems that are difficult to eradicate at a medical, industrial, or environmental level. Biofilms confer bacteria protection against external factors and antimicrobial treatments. Taking into account that about 80% of human infections are caused by bacterial biofilms, the eradication of these structures is a great priority. Biofilms are resistant to old-generation antibiotics, which has led to the search for new antimicrobials from different sources, including deep oceans/seas. In this study, 675 extracts obtained from 225 cyanobacteria and microalgae species (11 phyla and 6 samples belonging to unknown group) were obtained from different culture collections: The Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC), the Coimbra Collection of Algae (ACOI) from Portugal, and the Roscoff Culture Collection (RCC) from France. The largest number of samples was made up of the microalgae phylum Chlorophyta (270) followed by Cyanobacteria (261). To obtain a large range of new bioactive compounds, a method involving three consecutive extractions (hexane, ethyl acetate, and methanol) was used. The antibiofilm activity of extracts was determined against seven different bacterial species and two Candida strains in terms of minimal biofilm inhibitory concentration (MBIC). The highest biofilm inhibition rates (%) were achieved against Candida albicans and Enterobacter cloacae . Charophyta , Chlorophyta , and Cyanobacteria were the most effective against all microorganisms. In particular, extracts of Cercozoa phylum presented the lowest MBIC 50 and MBIC 90 values for all the strains except C. albicans . |
