[Expression of MiR101 and EZH2 in Patients with Mantle Cell Lymphoma and Its Clinical Significance].

Autor:	Lin YL; Department of Pathology,Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China., Zou ZK; Department of Pathology,Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China., Su HY; Department of Pathology,Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China., Huang YQ; Department of Hematology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China,E-mail: nchuangyiqun@126.com.
Jazyk:	čínština
Zdroj:	Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2019 Jun; Vol. 27 (3), pp. 820-826.
DOI:	10.19746/j.cnki.issn.1009-2137.2019.03.029
Abstrakt:	Objective: To investigate the expression of miR-101 and EZH2 in patients with mantle cell lymphoma(MCL) and to analyze its correlation with clinical prognosis of MCL patients. Methods: RQ-PCR and S-P immunohistochemistry were used to detect the expressions of miR-101 and EZH2 in tissue of MCL patients. CCK-8 was used to assay the effect of miR-100 minics on the proliferation of Jeko-1 and Mino cells; the flow cytometry with Annexin V/PI double staining was used to assay the apoptosis; Western blot was used to assay the effect of miR-101 minics on the expression of EZH2 protein in Jeko-1 and Mino cells. Results: Compared with control group, miR-101 lowly expressed, and EZH2 protein highly expressed in MCL group, with very statistically significant difference(P<0.01).There was negative correlation between miR-101 and EZH2 expression(r=-0.638，P<0.05). The expression of miR-101 and EZH2 significantly correlated with B symptoms, International Prognostic Index(IPI) and Ann Arbor stage, respectively. Survival analysis showed that the overall survival(OS) rate of patients with low expression of miR-101 were significantly lower than that of patients with high miR-101 expression (P=0.0014), the OS rate of patients with EZH2 high expression were significantly lower than that of patients with EZH2 low expression (P=0.0093). The miR-100 minics could inhibit the proliferation of Jeko-1 and Mino cells, and increase the apoptotic rate. The expression of EZH2 protein was markedly suppressed by the miR-100 minics. Conclusion: The expression of miR-101 and EZH2 is different in MCL patients with different clinical stage and prognosis. The miR-101 can inhibit the cell proliferation and induce cell apoptosis of mantle cell lymphoma by targeting EZH2.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu