Cryopreservation of the human gut microbiota: Current state and perspectives.

Autor: Smirnova DV; Center for Strategic Planning and Management of Medical and Biological Health Risks, Moscow, 119121, Russian Federation. Electronic address: S_mir_nova@mail.ru., Zalomova LV; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russian Federation., Zagainova AV; Center for Strategic Planning and Management of Medical and Biological Health Risks, Moscow, 119121, Russian Federation., Makarov VV; Center for Strategic Planning and Management of Medical and Biological Health Risks, Moscow, 119121, Russian Federation., Mezhevikina LM; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russian Federation., Fesenko EE Jr; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russian Federation., Yudin SM; Center for Strategic Planning and Management of Medical and Biological Health Risks, Moscow, 119121, Russian Federation.
Jazyk: angličtina
Zdroj: International journal of medical microbiology : IJMM [Int J Med Microbiol] 2019 Jul; Vol. 309 (5), pp. 259-269. Date of Electronic Publication: 2019 Jun 04.
DOI: 10.1016/j.ijmm.2019.06.001
Abstrakt: The human intestinal microbiota is a complex ecosystem that consists of thousands of bacterial species that are responsible for human health and disease. The intestinal microbiota is a natural resource for production of therapeutic and preventive medicals, such as probiotics and fecal transplants. Modern lifestyles have resulted in the extinction of evolutionally selected microbial populations upon exposure to environmental factors. Therefore, it is very important to preserve the human gut microbiota to have the opportunity for timely restoration with minimal safety risks. Cryopreservation techniques that are suitable for the preservation of viable, mixed microbial communities and a biobanking approach are currently under development in different countries. However, the number of studies in this area is very limited. The variety of morphological and physiological characteristics of microbes in the microbiota, the different cryopreservation goals, and the criteria for the evaluation of cryopreservation effectiveness are the main challenges in the creation of a universal and standardized cryopreservation protocol. In this review, we summarized the current progress of the main cryopreservation techniques for gut microbiota communities and the methods for the assessment of the effectiveness of these techniques in the context of practical application.
(Copyright © 2019 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: