| Autor: |
Casas-Junco PP; Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit, Mexico. mcapalomaa@outlook.es., Solís-Pacheco JR; Laboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingeniería, Universidad de Guadalajara, Boulevard Marcelino García Barragán #1421, Col. Olímpica, C.P. 44430 Guadalajara, Jalisco, Mexico. josuesolisp@gmail.com., Ragazzo-Sánchez JA; Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit, Mexico. arturoragazzo@hotmail.com., Aguilar-Uscanga BR; Laboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingeniería, Universidad de Guadalajara, Boulevard Marcelino García Barragán #1421, Col. Olímpica, C.P. 44430 Guadalajara, Jalisco, Mexico. agublanca@gmail.com., Bautista-Rosales PU; Centro de Tecnología de Alimentos, Universidad Autónoma de Nayarit, Ciudad de la Cultura 'Amado Nervo', C.P. 63155 Tepic, Nayarit, Mexico. u_bautista@hotmail.com., Calderón-Santoyo M; Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit, Mexico. montserratcalder@gmail.com. |
| Abstrakt: |
Ochratoxin A (OTA) produced by mycotoxigenic fungi ( Aspergillus and Penicillium spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid proliferation of mycotoxigenic fungi. Roasted coffee samples were artificially inoculated with A. westerdijikiae, A. steynii, A. versicolor , and A. niger , and incubated at 27 °C over 21 days for OTA production. Samples were cold plasma treated at 30 W input power and 850 V output voltage with helium at 1.5 L/min flow. OTA production in coffee was analyzed by high performance liquid chromatography coupled to a mass spectrometer (HPLC-MS). After 6 min of treatment with cold plasma, fungi were completely inhibited (4 log reduction). Cold plasma reduces 50% of OTA content after 30 min of treatment. Toxicity was estimated for extracts of artificially contaminated roasted coffee samples using the brine shrimp ( Artemia salina ) lethality assay. Toxicity for untreated roasted coffee was shown to be "toxic", while toxicity for cold plasma treated coffee was reduced to "slightly toxic". These results suggested that cold plasma may be considered as an alternative method for the degradation and reduction of toxin production by mycotoxigenic fungi in the processing of foods and feedstuffs. |
