| Autor: |
Ben Saad R; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. '1177' 3018, Sfax, Tunisia; and Corresponding author. Email: raniabensaad@gmail.com., Harbaoui M; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. '1177' 3018, Sfax, Tunisia., Ben Romdhane W; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. '1177' 3018, Sfax, Tunisia; and Plant Production Department, College of Food and Agricultural Sciences, King Saud University, PO Box 2460, 11451 Riyadh, Saudi Arabia., Zouari N; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. '1177' 3018, Sfax, Tunisia., Giang KN; International Joint Laboratory (LMI-RICE2), National Key Laboratory of Plant Cell Biotechnology, Agricultural Genetics Institute (AGI), Vietnam Academy of Agriculture Sciences (VAAS), Km2 Pham Van Dong Road, Co Nhue, Tu Liem District, Hanoi 10000, Vietnam., Ben Hsouna A; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. '1177' 3018, Sfax, Tunisia; and Department of Life Sciences, Faculty of Sciences of Gafsa, Zarroug 2112, Gafsa, Tunisia., Brini F; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. '1177' 3018, Sfax, Tunisia. |
| Abstrakt: |
Plant annexins are proteins with multiple functions and roles in plant development and responses to abiotic stresses. We report here the functional analysis of the TdAnn12 annexin protein isolated from Triticum durum Desf. We have previously shown that TdAnn12 expression is highly induced by different abiotic stresses. In the present study, to investigate the physiological and biochemical stress-induced responses, we overexpressed TdAnn12 in tobacco. We demonstrate that transgenic tobacco plants expressing TdAnn12 exhibited enhanced tolerance to salt, osmotic stress and H2O2 at the seedling stage. Under greenhouse conditions, these plants showed tolerance to drought and salt stresses. Moreover, scavenging reactive oxygen species (ROS), higher chlorophyll content, lower lipid peroxidation levels and increased antioxidant activities (peroxidase, catalase and superoxide dismutase) were observed. Finally, accumulation of TdAnn12 in tobacco positively affects the regulation of some stress-related genes (MnSOD, APX1, CAT1, P5CS, NHX1, SOS1 and DREB1A). TdAnn12 interacts directly or indirectly with stress-related genes that could stimulate an adaptive potential to gain tolerance which is not present in non-transgenic (NT) plants. Our results clearly show that overexpression of TdAnn12 in transgenic tobacco improves stress tolerance through the removal of ROS. |
