Evolutionary transcriptomics reveals the origins of olives and the genomic changes associated with their domestication.

Autor: Gros-Balthazard M; AGAP, University Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France., Besnard G; Laboratoire EDB UMR5174, CNRS-UPS-IRD, Toulouse, France., Sarah G; AGAP, University Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France., Holtz Y; AGAP, University Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France., Leclercq J; AGAP, University Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France., Santoni S; AGAP, University Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France., Wegmann D; Department of Biology, University of Fribourg, Fribourg, Switzerland.; Swiss Institute of Bioinformatics, Fribourg, Switzerland., Glémin S; CNRS, Université de Rennes, ECOBIO (Ecosystèmes, biodiversité, évolution) - UMR 6553, F-35000, Rennes, France.; Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden., Khadari B; AGAP, University Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France.; Conservatoire Botanique National Méditerranéen, UMR AGAP, Montpellier, France.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2019 Oct; Vol. 100 (1), pp. 143-157. Date of Electronic Publication: 2019 Jul 11.
DOI: 10.1111/tpj.14435
Abstrakt: The olive (Olea europaea L. subsp. europaea) is one of the oldest and most socio-economically important cultivated perennial crop in the Mediterranean region. Yet, its origins are still under debate and the genetic bases of the phenotypic changes associated with its domestication are unknown. We generated RNA-sequencing data for 68 wild and cultivated olive trees to study the genetic diversity and structure both at the transcription and sequence levels. To localize putative genes or expression pathways targeted by artificial selection during domestication, we employed a two-step approach in which we identified differentially expressed genes and screened the transcriptome for signatures of selection. Our analyses support a major domestication event in the eastern part of the Mediterranean basin followed by dispersion towards the West and subsequent admixture with western wild olives. While we found large changes in gene expression when comparing cultivated and wild olives, we found no major signature of selection on coding variants and weak signals primarily affected transcription factors. Our results indicated that the domestication of olives resulted in only moderate genomic consequences and that the domestication syndrome is mainly related to changes in gene expression, consistent with its evolutionary history and life history traits.
(© 2019 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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